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中文摘要：
研究了依思明蓝和牛血清白蛋白结合的共振散射光谱特征．实验结果表明，在pH=3．78水溶液中，血清白蛋白可与染料探针依思明蓝通过疏水作用结合并产生以367．4nm为特征峰的共振光散射（RLS）增强光谱．在该特征波长下测定的增强共振光散射强度（△IRLs）与血清白蛋白浓度在一定范围内具有线性关系．据此建立了痕量血清蛋白的共振光散射分析法．当依思明蓝的浓度为1．5×10^-5mol／L时，牛血清白蛋白，人血清白蛋白以及IgG的检测限分别可达2．34ng／mL，6．86ng／mL和10．9ng／mL．基于共振光散射数据及Scatchard图，获得依思明蓝与牛血清白蛋白和人血清白蛋白的结合常数（K）以及结合位点数（n）分别为2．90×10^5L／mol，2.9和2．96×10^5L／mol，0．35。
英文摘要：
Studies on binding of Isamin Blue （IB） and BSA are presented here. At pH=3.78, the static interaction of IB with BSA occurs and results in greatly enhanced resonance light scattering（RLS） signals characterized by a peak at 367.4 nm. It was found that the enhanced RLS intensity （△IRLS） at 367.4 nm is proportional to the concentration of BSA, and thus a new method for trace BSA analysis could be estab lished accordingly. The limit of determination （3σ） is 2.34 ng/mL when 1.5×10^-5 mol/L IB was em ployedfor BSA, 6.86 ng/mL for HSA and 10.9 ng/mL for IgG, respectively. Based on the measurements of the RLS data, a Scatchard plot concerning the interaction between BSA-IB and HSA-IB could be constructed and the binding number （n） and binding constant （K） are 2.9, 2.90×10^5 L/mol and 0.35, 2.96× 10^5 L/mol, respectively.
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