中文摘要：

为了研究法尼酸甲基转移酶（FAMeT）在甲壳动物蜕皮调控中的作用,采用反转录PCR（RT-PCR）和cDNA末端快速扩增技术（RACE）克隆得到了三疣梭子蟹FAMeT的全长cDNA序列（GenBank登录号：KC192659）,它包括一个201 bp的5＇非编码区、一个318 bp的3＇非编码区和一个825 bp的开放阅读框（ORF）,编码274个氨基酸;推导的氨基酸序列与已公布的其他甲壳动物FAMeT进行比对,发现一致性达75%～97%,其中与远海梭子蟹FAMeT的一致性最高;而且该氨基酸序列由两个CF（CPAMD8/FAMeT）区域组成,这两个CF区域是FAMeT的标志,在所有甲壳动物的FAMeT里均有发现,因此推导的氨基酸序列是三疣梭子蟹FAMeT基因。运用实时荧光定量PCR（qRT-PCR）的方法分析了其在不同组织中、不同蜕皮周期中的表达量变化,发现FAMeT在三疣梭子蟹的各个组织里均有表达,且在胸神经节里表达最强;在三疣梭子蟹蜕皮过程中,大颚器中FAMeT在D1期表达最强,然后逐渐下降至D4最低。该结果表明FAMeT在三疣梭子蟹蜕皮调控中起着重要的作用。

英文摘要：

To study the regulatory role of farnesoic acid O-methyl transferase（FAMeT） in molting process of crustaceans, the full-length FAMeT cDNA （GenBank accession number： KC192659 ） of Portunus trituberculatus is cloned by using reverse transcript PCR （RT-PCR） and rapid-amplification of cDNA ends （ RACE ）. FAMeT cDNA contains a 201 bp 5＇-untranslated region （ 5＇-UTR）, a 318 bp 3 ＇-untranslated region （3＇-UTR） and a 825 bp opening reading frame, which encodes 274 amino acid residues. Alignment of deduced amino acid sequence with FAMeT amino acid sequences of other crustaceans revealed that it shares the highest identity with P. pelagicus among the identities ranging from 75% to 97%. The amino acid residues consist of two copies of CF（CPAMD8/FAMeT） domain, which are the hallmark domain of FAMeT and are present in all crustacean FAMeTs. Quantitative real-time PCR（qRT-PCR）was used to quantify the relative expression level of FAMeT in different tissues and at molting stages in P. trituberculatus. The results showed that FAMeT was expressed in various tissues. The mRNA level of FAMeT was the highest in taoracic ganglia, followed by gill and mandibular organ （MO）. During the molting process, the expression of FAMeT in MO increased to the maximum at D~ stage, then gradually decreased to the minimum at D4 stage. The results suggest that FAMeT plays an important role in molting regulation in P. trituberculatus.