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中文摘要：
为明确黄淮麦区小麦品种（系）的遗传基础,采用SRAP（Sequence-related Amplified Polymorphism,相关序列多态性扩增）分子标记检测70份黄淮麦区小麦品种（系）的遗传多样性。结果表明,SRAP引物可产生清晰条带,47对引物组合检测出1 144个等位变异,其中具有多态性314个,多态性条带比列为27.5%,每对引物平均检测出6.68个多态性等位变异。SRAP引物的多态信息含量（PIC）为0.144 5～0.686 3,平均值为0.471 7。黄淮麦区中,河南省小麦品种（系）的多样性指数最高（0.584）,与其他各省遗传距离最近（0.145）,基因交流最多。江苏省小麦品种（系）多样性指数最低（0.366）,与其他各省遗传距离最远（0.250）,基因交流最少。聚类分析表明,遗传相似系数为0.334～0.801,平均值为0.596。供试材料可分为3大类5个亚类,群体遗传结构分析与聚类结果基本一致,SRAP分子标记可用于小麦遗传多样性检测和群体结构的判断和划分,可高效揭示种质资源的遗传背景和亲缘关系。
英文摘要：
The genetic diversity of 70 wheat cultivars from Huang-Huai wheat area of China was analyzed using SRAP-PCR method to investigate the genetic basis of wheat varieties in this area.The results showed that 47 pairs of SRAP primer combinations produced 1144 bands,of which 314 bands were polymorphic with 27.45% of polymorphism,average 6.68 polymorphic bands per primer pair.The range of polymorphic information content（PIC）was from 0.144 5to 0.686 3,with an average of0.471 7.Henan wheat cultivars had the highest genetic diversity index（0.584）and the smallest genetic distance（0.145）among the six provinces,which meant the most gene connections with other provinces.Conversely,Jiangsu had the least gene connection.The cluster analysis divided the accessions into three group and five subgroups,and the GS ranged from 0.334 to 0.801,with an average of0.596.The structure analysis divided the accessions into five groups,which was similar to the result of clusting analysis.These results imply that SRAP might be a potentially useful marker technique for evaluating genetic diversity and dividing group structure in wheat cultivars,and revealing the background and genetic relationship between germplasm resources efficiently.
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