钾和硼均是植物必需的营养元素,对植物的生长、发育与生理代谢等起着重要作用。棉花对钾和硼都有较高的需求量,而棉花缺钾或缺硼时有发生。以鄂抗8号品种的棉花作为研究材料,利用傅里叶变换探讨钾硼胁迫对棉花功能叶片物质成分的影响。红外光谱(FTIR)对钾硼胁迫下棉花叶片的物质成分进行研究,分析钾硼缺乏情况下叶片红外光谱的差异。结果表明:(1)缺钾处理时,1 546.86,1 438.85,1 153.39和1 024.17cm-1这4个特征峰消失,且其他特征峰的相对吸光度值与正常处理相比均减小,表明缺钾时棉花功能叶片蛋白质、纤维素、可溶性糖和核糖的结构发生变化且含量降低。(2)缺硼条件下,叶片的特征峰的相对吸光度均有所升高,因而,缺硼则导致蛋白质及可溶性糖、纤维素等碳水化合物等的运输受阻而在叶片中积累。(3)钾硼同时缺乏,棉花功能叶片特征峰的位置和相对吸收强度与正常处理相比有很大差异,表现为棉花功能叶片蛋白质和可溶性糖等碳水化合物的含量升高,而核酸和多糖分子等含量下降。因此缺钾导致棉花功能叶片蛋白质、纤维素、可溶性糖和核糖结构变化的含量降低;缺硼时蛋白质及可溶性糖、纤维素等碳水化合物等在叶片中积累;缺钾缺硼时蛋白质和可溶性糖等碳水化合物含量升高,而核酸和多糖分子等含量下降。
Potassium(K)and boron(B)are essential nutrient elements for plants,and the elements play an important role for plant growth,development and physiological metabolism.Cotton has a higher demand for K and B;K deficiency or B deficiency often occurs in cotton though.To reveal the component changes in functional leaf of cotton under K and B stress and investigate effects on material composition from K and B.A pot experiment was conducted at Huazhong Agricultural University.(1)the characteristic peaks at 1 546.86,1 438.85,1 153.39 and 1 024.17cm-1 disappeared due to B deficiency,and relative absorbance of other characteristic peaks was decreased compared with normal,which suggested that the structures of protein,fiber,soluble sugar and ribosome in cotton functional leaf changed and decreased in cotent when lack of K.(2)the relative absorbance of all characteristic peaks was increased in the B-deficient cotton leaves compared with normal,suggesting B deficiency leads to the accumulation in leaves of protein,and fiber,soluble sugar and other carbohydrates because of the hindered transportation.(3)lack of both potassium and boron,induced significant changes to both the locations and relative absorbance of characteristic peaks,and the content of protein,and soluble sugar and other carbohydrates increased,while the content of nucleic acids and polysaccharides dropped.K deficiency led to the structures of protein,fiber,soluble sugar and ribosome in cotton functional leaf changed and decreased in content;B deficiency gave rise to the accumulation in leaves of protein,and fiber,soluble sugar and other carbohydrates;the content of protein and soluble sugar and other carbohydrates increased,while the content of nucleic acids and polysaccharides dropped when K and B were all in short supply.