中文摘要：

目的：建立HPLC法同时测定柴胡和春柴胡中2个皂苷类活性成分（柴胡皂苷a和柴胡皂苷d）、4个黄酮类活性成分（芦丁、槲皮素、山柰素和异鼠李素）的含量。方法：采用Agilent Zorbax C18（4.6 mm×250 mm,5μm）色谱柱,以0.1%磷酸水-乙腈为流动相,梯度洗脱,流速1 mL·min^-1,检测波长203 nm（测定皂苷类成分）和360 nm（测定黄酮类成分）,柱温35℃。结果：6个活性成分的峰面积与浓度的线性关系良好（r≥0.9994）,线性范围宽;精密度试验的RSD为0.67%-1.9%;加样回收率为95.1%-104.3%。10批市售柴胡和春柴胡的黄酮类和皂苷类成分含量具显著差异：春柴胡中4个黄酮类成分平均含量约为柴胡中的7.5-72倍;春柴胡中柴胡皂苷a的平均含量为柴胡的1/10,而柴胡皂苷d的平均含量约为柴胡的3倍;柴胡与春柴胡中6个成分含量的相关系数为0.10-0.62。结论：本方法具良好的精密度、重复性和可靠性,可同时测定柴胡和春柴胡中皂苷类和黄酮类成分的含量并有效表征其差别。

英文摘要：

Objective：To establish an HPLC method for the simultaneous determination of 2 saponins（saikosaponin a,saikosaponin d）and 4 flavonoids（rutin,quercetin,kaempferide and isorhamnetin）of Bupleuri Radix and Bupleuri Herba.Methods：These six compounds were analyzed simultaneously with an Agilent Zorbax C18（4.6 mm×250 mm,5 μm）column by gradient elution using 0.1%（v/v）phosphoric acid-acetonitrile as the mobile phase at the flow rate of 1 mL·min-1;The detection wavelength was set at 203 nm for saponins and 360 nm for flavonoids,and the column was maintained at 35 ℃.Results：All the compounds showed good linearity（r≥0.9994）in a relatively wide concentration range.RSDs of the precision test were 0.67%-1.9%,and the recoveries were 95.1%-104.3%.The overall reproducibility of the method was acceptable.There was significant difference on content of saponins and flavonoids between Bupleuri Radix and Bupleuri Herba：content of flavonoids in Bupleuri Herba was about 7.5-72 times of that in Bupleuri Radix;while content of saikosaponin a in Bupleuri Radix was about 10 times of that in Bupleuri Herba,content of saikosaponin d in Bupleuri Herba was about 3 times of that in Bupleuri Radix;Correlation of content between Bupleuri Herba and Bupleuri Radix was 0.10-0.62.Conclusion：The validated HPLC-DAD method has the advantages of precision,reproducibility and reliability,allowing simultaneous determination and comparison of saponins and flavonoids between Bupleuri Radix and Bupleuri Herba.