中文摘要：

目的：采用荷瘤动物模型经化疗联合被动免疫治疗,初步探讨化疗免疫“窗口期冶对卵巢癌免疫应答的影响及其可能的免疫学机制。方法：以荷瘤大鼠为研究对象,根据紫杉醇联合卡铂对荷瘤鼠免疫功能改变的不同时期给予CTL（cytotoxic T-lympho鄄cyte）主动免疫治疗。观察荷瘤体积及机体免疫微环境改变。结果：荷瘤鼠紫杉醇和卡铂化疗后不同时期给予CTL转输治疗,其中化疗后第6天即淋巴细胞数最少期,给予免疫治疗,肿瘤生长最缓慢;CT扫描结果示,化疗后6天＋免疫治疗组的瘤体积最小,且与其余治疗组有统计学意义。荷瘤鼠免疫功能示,化疗后6天＋免疫治疗组中淋巴细胞抗原特异性增殖及CD8＋T细胞最明显,而Treg（T-Lymphocytes,Regulatory）细胞明显降低。结论：紫杉醇和卡铂联合CTL治疗肿瘤具有协同作用,其中化疗后6天（即淋巴细胞降到最低期）是CTL免疫治疗的最佳时间点。化疗造成的肿瘤个体免疫功能的低下或是免疫系统空间的释放（所谓的免疫“窗口期冶）为诱导特异性的抗肿瘤免疫应答提供了可能,免疫状态的检测是保证肿瘤患者化疗联合免疫治疗方案治疗有效性的重要前提之一。

英文摘要：

Objective：To evaluate the influence of synergistic antitumor activity on the immunologic response of ovarian cancer,and investigate the immunologic mechanism behind it, by applying chemotherapy combined with immunotherapy on the treatment of rat-bearing tumors. Methods：By applying adoptive immunotherapy with CTL to rat-bearing tumors at differ-ent time of the change of immunocyte number and immune function from established tumor treatment with paclitaxel and carboplatin chemotherapy, observe the changes of the volume of the established tumor and the immune function of different groups. Results：The combination at the 6th post-chemotherapy day with CTL led to the most significantly delayed tumor growth. In this group,the number of CD8＋T cell was the highest,while the expression of Treg cell was the lowest. Conclusions：Paclitaxel and carboplatin chemotherapy has synergistic effects with adop-tive transfer of CTLs. It is optimal to apply the immune therapy against tumors at 6 days after chemotherapy. The lowest number of lymphocytes in the animal periphery might represent the optimal checkpoint for the immune therapy. Therefore,monitoring the immune status of tumor patients might become one of the important prerequisites for effective immune therapy when de-signing the comprehensive therapeutic strategies.