中文摘要：

目的：探索卡波济肉瘤相关疱疹病毒（Kaposi’s sarcoma-associated herpesvirus,KSHV）体外感染人牙龈成纤维细胞（human gingival fibroblast,HGF）的途径和方法,为研究KSHV导致口腔卡波济肉瘤病变的机制提供依据。方法：用12-O-十四烷酰佛波醇-13-乙酯（12-O-tetradecanoylphorbol-13-acetate,TPA）刺激人原发性渗出性淋巴瘤（primary effusion lymphoma,PEL）细胞系的BC-3细胞,收集细胞上清（含KSHV病毒颗粒）,感染HGF细胞。观察细胞病变效应（cytopatric effect,CPE）,采用RT-PCR和Western blot分别检测HGF细胞内KSHV编码基因的转录情况和蛋白表达水平。结果：KSHV感染HGF细胞后可出现CPE;感染后采用RT-PCR可检测到不同时间点ORF26和ORF73基因的转录;感染后12 h可检测到vIL-6蛋白的表达。结论：KSHV可感染HGF细胞并建立潜伏感染,为进一步研究KSHV导致的口腔KS发病机制提供了较好的体外模型。

英文摘要：

To establish a model of infecting human gingival fibroblast（HGF）cells with KSHV in vitro for further studying the mechanism of oral KS pathogenesy.Methods：The supernatants containing KSHV virion harvested from primary effusion lymphoma（PEL） cells BC-3 were used to infect HGF cells.The cytopathic effect（CPE） of HGF cells was observed post-infection and the lytic replication of KSHV in HGF cells was detected by RT-PCR and Western blot.Results：CPE appeared in post-infective HGF cells.KSHV ORF26 and ORF73 mRNA transcription was detected in HGF cells by RT-PCR at diffenrent time points.And the specific band of vIL-6 protein was detected at 12 hours post-infection by Western blot.Conclusion：KSHV could infect HGF cells and establish latent infection,providing a model in vitro for further studying the mechanism in KSHV leading to oral KS.