中文摘要：

三维荧光光谱广泛应用于溶解性有机物（DOM）的表征,然而,荧光图谱中多种荧光组分相互重叠使得荧光激发发射矩阵数据难以进一步处理,导致对相应的荧光组分定性定量表征、来源追溯及与特定环境行为之间相关关系的分析造成困难。综述了传统峰值法、荧光区域积分法及平行因子分析法等三维荧光光谱的数据分析方法,并对近年来发展的高效液相色谱/高效体积排阻色谱与平行因子联用的方法进行了介绍,在此基础上比较总结了各方法的优缺点及适用范围,展望了三维荧光光谱分析的发展方向。

英文摘要：

Three-dimensional fluorescence spectroscopy has been widely applied in the characterization of dissolved organic matter （DOM）. The complicated overlapping between a variety of fluorescent substances in the fluorescence excitation- emission matrix dataset is difficult to further qualitatively and quantitatively analyze the characterization, source and the correlations of fluorescent components with specific environmental behaviors.Several data analysis methods for the excitation- emission matrix spectrum, including traditional peak-picking, fluorescence regional integration （FRI） and parallel factor analysis（PARAFAC） were reviewed. In addition, a latest study on the combination of high performance liquid chromatography （HPLC）/high-performance size-exclusion chromatography （HPSEC） with fluorescence and PARAFAC was also introduced. Meanwhile a comprehensive comparison was conducted for these methods. The development direction of three-dimensional fluorescence spectroscopy was also prospected.