中文摘要：

抗生素滥用所导致的健康问题已成为人们关注的焦点。为了快速检测抗生素残留,利用荧光法对鸭肉中新霉素的残留进行检测。在乳化剂OP-10的环境下,新霉素和邻苯二甲醛、巯基乙醇发生化学反应,经过激发光照射后可以产生强的荧光。以新霉素为研究对象,研究了其自身和反应物的荧光强度。研究结果表明：加入1.5 mL邻苯二甲醛溶液,1 mL乳化剂OP-10溶液,2.5 mL巯基乙醇溶液,并且反应90 min后测得的荧光强度为最佳;对浓度范围在1.00～8.00μg/mL的新霉素标准样品进行测定,得到的线性方程为,相关系数为0.984 9;对含不同浓度新霉素的鸭肉提取液进行测定,相关系数为0.999 8。结果表明,荧光法是一种方便、快速的新霉素残留分析方法。

英文摘要：

Health problems caused by overuse of antibiotics have become the focus of attention.In order to detect antibiotic residues rapidly;fluorescence method was used to detect the neomycin residues in duck.A chemical reaction of neomycin with ortho-phthalaldehyde,mercaptoethanol was involved in the emulsifier OP-10 environment and strong fluorescence was produced after excitation light irradiation.With neomycin as the object of the study,fluorescence intensity of its own and the reactant was studied.The results indicated that the best fluorescence intensity was measured after 90 min by adding 1.5 mL o-phthalaldehyde solution,1 mL emulsifier OP-10 solution,2.5 mL mercapto ethanol solution;Neomycin standard samples of concentration range in 1.00-8.00 μg/mL were measured to obtain the linear equation F=45.3C＋9.7,thecorrelation coefficient was 0.984 9;duck extracted solution with different concentrations of neomycin was determined,the correlation coefficient was 0.9995.The results showed that the fluorescence method was a convenient and rapid analytical method for detection of neomycin residue.