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Increased heat shock protein 70 expression in the pancreas of rats with endotoxic shock
  • ISSN号:1007-9327
  • 期刊名称:《世界胃肠病学杂志:英文版》
  • 时间:0
  • 分类:R576[医药卫生—消化系统;医药卫生—临床医学;医药卫生—内科学]
  • 作者机构:[1]Department of Infectious Diseases, The Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China, [2]Department of Internal Medicine, the Fourth People's Hospital of Shenyang, Shenyang 110007, Liaoning Province, China, [3]Department of Infectious diseases, the First Affiliated Hospital of Jinzhou Medical College, Jinzhou 121004, Liaoning Province, China
  • 相关基金:Supported by the National Natural Science Foundation of China, No. 30170849
中文摘要:

瞄准:调查极端结构的变化并且加热吃惊蛋白质 70 (HSP70 ) 在有内毒素的老鼠的胰的表示震惊并且检测他们的可能的关系。方法:33 只 Wistar 老鼠的一个总数随机被划分成三个组:控制组(给定的生理盐水) ,脂肪的多糖(LPS ) 组织的小剂量(给定的 LPS 5 mg/kg ) 和大剂量 LPS 组(给定的 LPS 10 mg/kg ) 。胰是 explanted 由免疫组织化学和西方的污点由 TEM 和 HSP70 表示检测极端结构的变化。结果:LPS 的给定的小剂量显示出胀大的老鼠和腺泡房间的 mitochondrial 嵴和在腺泡房间的细胞质的 autophagic 液泡的增加的数字的损失。LPS 的给定的大剂量显示出胀大的老鼠,空泡形成,和腺泡房间的 mitochondrial 嵴的明显的骨髓的变化,在腺泡房间的细胞质的 autophagic 液泡的增加的数字。HSP70 表示与控制组(P【0.05 ) 相比被增加。结论:LPS 的小剂量可以导致 HSP70 的更强壮的表示,支持汽车吞噬作用并且改善极端结构的损害。

英文摘要:

AIM: To investigate the ultra-structural changes and heat shock protein 70 (HSP70) expression in the pancreas of rats with endotoxic shock and to detect their possible relationship. METHODS: A total of 33 Wistar rats were randomly divided into three groups: control group (given normal saline), small dose lipopolysaccharide (LPS) group (given LPS 5 mg/kg) and large dose LPS group (given LPS 10 mg/kg). Pancreas was explanted to detect the ultrastructural changes by TEM and the HSP70 expression by immunohistochemistry and Western blot. RESULTS: Rats given small doses of LPS showed swelling and loss of mitochondrial cristae of acinar cells and increased number of autophagic vacuoles in the cytoplasm of acinar cells. Rats given large doses of LPS showed swelling, vacuolization, and obvious myeloid changes of mitochondrial cristae of acinar cells, increased number of autophagic vacuoles in the cytoplasm of acinar cells. HSPT0 expression was increased compared to the control group (P〈0.05). CONCLUSION: Small doses of LPS may induce stronger expression of HSP70, promote autophagocytosis and ameliorate ultra-structural injuries.

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  • 《世界胃肠病学杂志:英文版》
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  • 国际标准刊号:ISSN:1007-9327
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  • 被引量:12408