中文摘要：

以分离自南方红豆杉根际的1株解有机磷细菌JYD-4为研究对象,对其进行分类鉴定;采用钼锑抗比色法研究JYD-4菌种在不同碳源、氮源和pH条件下的解有机磷能力;提取JYD-4菌株磷酸酯酶,测定在不同温度和pH作用下的磷酸酯酶活性;采用温室盆栽试验研究JYD-4菌株对南方红豆杉实生苗的促生长作用。结果表明：（1）分离自南方红豆杉根际的解有机磷细菌JYD-4为嗜麦寡养单胞菌（Stenotrophomonas maltophilia）。（2）菌株JYD-4在卵黄固体培养基上产生的解磷圈与菌落直径比为2.01,在液体培养基中的解磷量为72.38mg/L。（3）菌株JYD-4解磷最适碳源为葡萄糖,最适氮源为牛肉膏,最适pH 7.0。（4）菌株JYD-4产生的磷酸酯酶主要为胞内酶,该酶在20℃~65℃温度范围均能发挥较高酶活性,但仅能在pH 9.0条件下产生较高酶活性。（5）菌株JYD-4接种能够明显提高南方红豆杉实生苗的苗高、地径和生物量。研究表明,嗜麦寡养单胞菌JYD-4是一株高效解有机磷细菌,主要分泌碱性磷酸酯酶,对南方红豆杉具有明显的促生长作用;该研究为南方红豆杉微生物肥料的开发提供了优良的菌种资源,为该菌种的进一步开发应用提供了理论依据。

英文摘要：

We isolated and identified an organophosphate-solubilizing bacterium JYD-4from the rhizosphere of Taxus chinensis var.mairei.Molybdenum-antimony anti-spectrophotometric method was applied to investigate the effects of carbon sources,nitrogen sources and pH environments on organophosphate solubilization capacity.We also detected the effects of temperatures and pH on the phosphatase enzyme activity produced by strain JYD-4.The inoculation test in potted seedlings was carried out under the greenhouse.The results showed that：（1）strain JYD-4was identified as Stenotrophomonas maltophilia.（2）The ratio of organophosphate-solubilizing circle diameter to colony diameter was 2.01.Organophosphate-dissolving ability in liquid medium was 72.38mg/L.（3）Glucose and beef extract were identified as the optimal carbon source and nitrogen source for organophosphate-solubilizing.The optimal pH was 7.0.（4）Phospha-tase produced by S.maltophiliaJYD-4mainly located in the cell.The phosphatase could maintain stable activity in a wide temperature range（20-65℃）,only conferred high activity at pH 9.0.（5）S.maltophiliaJYD-4was able to improve the seedling height,ground diameter and biomass of the seedlings of T.chinensis var.mairei.The results showed that S.maltophilia JYD-4is a highly effective organophosphate-solubilizing bacterium.It could significantly promote the growth of Taxus chinensis var.mairei.Results in this paper provide excellent strain for the development of microbial fertilizer of T.chinensis var.mairei,and provide theory evidences for the application of S.maltophiliaJYD-4.