中文摘要：

Chlamydia trachomatis 是最流行的性播送的病原体之一。Chlamydial 主要的外部膜蛋白质(MOMP ) 能在鼠科的模型导致强壮的细胞、体液的有免疫力的回答并且被认为是一个潜在的疫苗的候选人。在这份报告， MOMP 的氨基酸顺序用帮助计算机的技术被分析扫描 B 房间 epitopes，和三可能的线性 B 房间 epitopes 肽(VLKTDVNKE， TKDASIDYHE， TRLIDERAAH ) 与高预言的 antigenicity 和高度，保存被调查。为各潜在的 epitope 编码区域的 DNA 被克隆进 pET32a (+) 并且在 Escherichia coli 表示了为 Trx-His-tag 熔化蛋白质。熔化蛋白质被 Ni-NTA agarose 祷告净化并且由 SDS 页和西方的污点分析列在后面。我们与这三熔化蛋白质使老鼠免疫。从使免疫的老鼠包含 anti-epitope 抗体的 sera 能认出 C。在 ELISA 的 trachomatis serovars D 和 E。这些熔化蛋白质的 Antisera 在 serovar E 的侵略上显示了禁止的效果由在 vitro 中立化试金。另外，浆液从恢复健康的 C 取样。感染 trachomatis 的病人与由西方的污点试金的 epitope 熔化蛋白质是反应的。我们的结果证明生物信息的分析选择的 epitope 序列是高度保存的 C。trachomatis MOMP B 房间 epitopes，并且能是子单元疫苗的发展的好候选人，它能在临床的诊断被使用。

英文摘要：

Chlamydia trachomatis is one of the most prevalent sexually transmitted pathogens. Chlamydial major outer membrane protein （MOMP） can induce strong cellular and humoral immune responses in murine models and has been regarded as a potential vaccine candidate. In this report, the amino acid sequence of MOMP was analyzed using computer-assisted techniques to scan B-cell epitopes, and three possible linear B-cell epitopes peptides （VLKTDVNKE, TKDASIDYHE, TRLIDERAAH） with high predicted antigenicity and high conservation were investigated. The DNA coding region for each potential epitope was cloned into pET32a（＋） and expressed as Trx- His-tag fusion proteins in Escherichia coli. The fusion proteins were purified by Ni-NTA agarose beads and followed by SDS-PAGE and western blot analysis. We immunized mice with these three fusion proteins. The sera containing anti-epitope antibodies from the immunized mice could recognize C. trachomatis serovars D and E in ELISA. Antisera of these fusion proteins displayed an inhibitory effect on invasion of serovar E by in vitro neutralization assays. In addition, serum samples from convalescent C. trachomatis-infected patients were reactive with the epitope fusion proteins by western blot assay. Our results showed that the epitope sequences selected by bioinformatic analy- sis are highly conserved C. trachomatis MOMP B-cell epitopes, and could be good candidates for the development of subunit vaccines, which can be used in clinical diagnosis.