中文摘要：

目的：探讨叶酸补充拮抗5,10-亚甲基四氢叶酸还原酶（MTHFR）基因下调的作用机制。方法：MTT法检测叶酸梯度补充后拮抗MTHFR基因功能下调对细胞增殖的影响;流式细胞仪分析MTHFR基因功能下调补充叶酸前后细胞周期的改变。结果：MTHFR基因功能下调后,不同剂量叶酸补充可以逆转MTHFR基因功能下调对EPM细胞的不良影响,影响程度与补充叶酸的浓度在一定范围内具有剂量依赖性。结论：MTHFR基因是重要的先天性唇腭裂候选基因,叶酸补充可以拮抗MTHFR基因功能下调对胚胎腭突发育的不良影响。

英文摘要：

Objective： To further understand the role of folic acid supplements rivaling MTHFR gene silencing in pathogenesis of NCLP,RNA interference（RNAi） was applied to knock down MTHFR in mouse embryonic palatal mesenchymal（EPM） cells.Methods： MTHFR ShRNA expression vector were transfected into the primary cultured EPM cells.MTT was used to observe cell proliferation after MTHFR gene silencing.FCM was used to observe cell cycle after MTHFR gene silencing.Results： The results showed the cells proliferation had an inequality amelioration after using folic acid supplements in MEPM cells with MTHFR gene silencing.Using folic acid supplements rivaled the effect of MTHFR gene silencing had a dose-dependent manner.Using 20 μg/ml folic acid supplements could improve the cell proliferation to achieve normal level of cell proliferation.Conclusion： MTHFR gene is an important candidate gene of NCL/P.Using folic acid supplements could prevent teratogenic MTHFR gene silencing for embryonic palate development.