通过盆栽试验研究了解毒剂酒石酸与螯合剂EGTA的单施与配施对强化蓖麻修复Cd污染土壤的作用,探讨重金属污染土壤植物修复中螯合剂与解毒剂配合使用的可行性。结果显示:(1)除酒石酸单施处理外,其余处理均可显著提高土壤中醋酸提取态Cd含量,增强土壤Cd的活性,并以酒石酸与EGTA配施的效果更显好,其土壤醋酸提取态Cd含量为对照的1.41~2.49倍。(2)EGTA能有效促进Cd从蓖麻根部向地上部的转移,但高剂量EGTA处理对蓖麻根系有明显的毒害作用;EGTA与酒石酸配合施能缓解Cd对植株的毒害作用,增大蓖麻生物量和Cd积累量,其地上部Cd积累量比对照增加4.56~8.32倍。(3)蓖麻叶片Cd含量、地上部积累总量以及土壤净化率随土壤醋酸提取态Cd含量的升高而增大,并且呈良好的线性递增关系。研究表明,酒石酸与EGTA配施可通过调控土壤Cd的植物可利用性和降低Cd的生理毒性来提高蓖麻对Cd的富集能力和对Cd污染土壤的修复效果。
A pot experiment was performed to study the effectiveness of tartaric acid and EGTA and their combined use in enhancing remediation of Cd contaminated soil with Ricinus communis L.,so as to discuss the feasibility of chelating agents and antidote in phytoremediation of heavy metal contaminated soil.The results showed that:(1)All treatments except single use of tartaric acid could significantly increase acetate-extractable Cd concentrations in soils.The acetate-extractable Cd concentrations in EGTA & tartaric acid treatment were 1.41~2.49 times higher than that of the control.(2)The Cd concentration in Ricinus communis L.shoots increased significantly with EGTA treatments,but high dose of EGTA demonstrated toxic effects on plant roots.The EGTA & tartaric acid treatments were more effective for Cd detoxication,translocation and biomass accumulation,which Cd accumulations in shoots are 4.56~8.32 times higher than that of control.As such,the phytoremediation efficiency is increased.(3)Statistical analysis reveals significant positive relations between the acetate-extractable Cd concentrations and the Cd content in leaves,the Cd accumulation in shoots,and cadmium removal rates in soil,respectively.Therefore,EGTA combined with tartaric acid can effectively improve Cd accumulation capacity in Ricinus communis L.shoots by adjusting Cd bioavailability and reducing its physiological toxicity.