中文摘要：

采用人工土壤法测定了不同浓度莫能菌素对蚯蚓的急性毒性和对蚯蚓生长、生存、繁殖以及超氧化物歧化酶（SOD）、谷胱甘肽-硫转移酶（GSH-S）、腺三磷酸酶（ATPase）、乙酰胆碱酯酶（T-CHE）活性的影响。结果表明,莫能菌素对蚯蚓14d的LC50为75.883mg·kg-1;当莫能菌素含量达到50mg·kg-1时显著影响蚯蚓的生存（P〈0.05）,死亡率达到20%;当莫能菌素含量达到25mg·kg-1时显著影响蚯蚓的生长（P〈0.05）;药物的染毒浓度与对蚯蚓生存和繁殖的抑制存在明显的剂量-效应关系。染毒21d后,低浓度的兽药莫能菌素就能显著影响蚯蚓体内SOD、GSH-S、ATPase、T-CHE的活性,药物浓度和酶活性间具有明显的剂量-效应关系。莫能菌素对土壤动物蚯蚓显示有毒性作用,对土壤环境具有潜在的生态风险。

英文摘要：

Effect of different concentration levels of monensin on earthworm were studied with an artificial soil test,including earthworm＇s growth,survival,production,and four kinds of enzyme activity of superoxide dismutase（SOD）,glutathione-sulfurtransferase（GSH-S）,acetylcholine esterase（T-CHE）and adenosine-triphosphatase（ATPase）.The results showed that LC50 of monensin was 75.883 mg·kg-1 after 14 days exposure.The reproduction of earthworms was significantly inhibited in lower monensin concentration（50 mg·kg-1）（P0.05）.The survival of earthworm was significantly affected at the monensin concentrations of 50 mg·kg-1（P0.05）with mortality of 20%.The growth of earthworms was inhibited at the monensin concentration of 25 mg·kg-1.There was a significant dose-response relation between the exposure concentration of monensin and earthworm growth and reproduction.In 21days＇ exposure,monensin affected significantly the enzymatic activities of SOD、GSH-S、T-CHE and ATPase,showing a significant dose-response relation between exposure concentration and enzyme activity.Monensin had direct toxic effects on soil animals and also existed with a potential ecological risk.