目的:制定蜘蛛香药材质量标准,为药用植物资源的开发利用提供科学依据。方法:生药学研究,浸出物测定法,灰分测定法,水分测定法,薄层色谱法及HPLC色谱法。结果:对蜘蛛香的性状、显微特征进行了描述;对15个不同产地及收集时间蜘蛛香的浸出物、总灰分、酸不溶灰分和水分进行了测定;同时对其活性成分缬草素和乙酰缬草素进行了薄层定性鉴别和HPLC定量研究。结论:通过研究制定了蜘蛛香的质量控制标准。
Objective: To provide scientific basis for the utilization and development of Valeriana jatamansi by setting up the quality control specification of V. jatamansi. Method: The pharmacognostical methods were applied. The extract of V. jatarnansi was examined. Moisture and ash were determined. And the bioactive constituents were analyzed by TLC and HPLC. Result: The morphological and histological characters of V. jatamansi were observed. Content of total ash, acid-insoluble ash, and moisture of 15 samples from different habitats and times were determined. The qualitative and quantitative analysis of valtrate and acevaltrate by TLC and HPLC were preformed respectively. Conclusion: The established method can be used for the quality control of V. jatamans.