中文摘要：

为了构建HA2基因的新功能型重组乳酸菌,本试验通过PCR将来源于H9N2亚型禽流感病毒的血凝素基因HA2插入锚定表达载体p SIP409-pgsA’中,构建重组质粒pSIP409-pgsA’-HA2,并将其电转到植物乳杆菌NC8中,通过W estern-blot检测目的蛋白的表达情况。结果显示,成功构建了NC8-pSIP409-pgsA’-HA2,经Western-blot检测目的蛋白被成功表达,并且具有反应原性,目的条带大小与目的蛋白一致,为后期试验奠定了基础。

英文摘要：

To construct novel functional lactic acid bacteria expressing HA2,the HA2 gene of avian influenza virus H9N2 subtype was amplified and inserted into anchoring vector p SIP409-pgsA＇, yielding pSIP409-pgsA＇-HA2.After transformation into Lactobacillus plantarum NC8 strain,the synthesis of HA2 protein was determined by Western-blot assay.The results indicated that the recombinant Lactobacillus plantarum NC8 with surface displayed HA2 protein of H9N2 subtype AIV was successfully constructed,with a specific protein band shown in Western-blot,providing the fundation for further studies.