中文摘要：

系统地研究了耐高温菌sulfolobus的生长特性．sulfolobus在65℃以上能良好地生长，并对亚铁和元素硫均具有较好的氧化作用；在75℃时，sulfolobus氧化Fe^2＋和元素硫的活性最强，细菌的生长曲线和细菌氧化Fe^2＋和元素硫的趋势一致．给出了细菌生长过程溶液SO4^2-和pH的变化趋势；以黄铜矿精矿为培养基对sulfolobus进行了适应性培养，并在75℃下进行了黄铜矿摇瓶浸出研究．结果显示：适应性驯化后的sulfolobus在黄铜矿精矿上生长与浸矿性能良好；当矿浆质量分数在10％以下，浸出150h，Cu浸出率可达90％以上；而矿浆质量分数在15％以上时，浸出350h，浸出率仅80％．同时还研究了浸出体系溶液氧化还原电位Eh和pH随时间的变化规律．

英文摘要：

The sulfolobus, a thermophilic microorganism, can grow at high temperature up to 65 ℃. It has good ability to oxidizing element sulfur and ferrous ion and can meet the high temperature demand in dump leaching and chalcopyrite stirring leaching in a reactor. The culture results show that at 75 ℃the sulfolobus has more adapted to the environment than below and above the temperature. The changing tends of the solution pH,redox potential, SO4^2- concentration and ferrous oxidation rate with time in bio culture as well as in bioleaching of chalcopyrite concentrate were elucidated. The bioleaching experiments show that using the trained sulfolobus strains at 75℃ in the shake blasting when the slurry mass fraction is below 10 %, the chalcopyrite concentrate leaching rate could up to 90 % after 150 h leaching. But when slurry mass fraction is up to 15 %, after 350 h leaching, the leaching rate is only 80 %. It demonstrates that the slurry mass fraction has apparently effect on the leaching results.