中文摘要：

目的：研究阳离子交换树脂分离和富集莲子心中黄酮与生物碱类物质的工艺条件。方法：以Nef、黄酮Ⅳ的吸附量及洗脱率为指标,对阳离子交换树脂的静态吸附-解吸性能、动态吸附-洗脱性能进行了考察。结果：药液比为1∶6的莲子心提取液经D152阳离子交换树脂吸附后,先用5BV纯化水洗去杂质,然后用10BV 70%乙醇洗脱,得黄酮类物质;用7BV 0.02 g/mL NaCl-70%乙醇洗脱,得生物碱类物质,两类物质洗脱率均在80%以上,精制倍数可分别达27.08倍及7.86倍。结论：该工艺能同时分离和富集莲子心中黄酮和生物碱类物质。

英文摘要：

Objective ： To explore the optimal separation condition of the total flavonoids and alkaloids from seed embryo of Nelum- bo nucifera. Methods： Types of cation exchange resins, concentration of extract liquid, solution volume, velocity of adsorption and eluent were evaluated in separating efficiency with measuring the adsorption ratio and eluent ratio. Results： D152 resin was finally chosen. After enrichment and purification with it, the purity of fiavonoids and alkaloids were significantly increased by 27.08 and 7. 86 times , respectively. Conclusion： This method is simple, feasible and suitable for the separation of total flavonoids and alkaloids from seed embryo of Nelumbo nucifera.