研究水稻花发育基因对于水稻相关性状的分子育种具有十分重要的意义。本研究报道了一个水稻颖壳和浆片异常突变体ahl(abnormal hull and lodicule),来源于优良恢复系缙恢10号的EMS诱变群体。该突变体内外稃变小并发生严重扭曲,浆片顶端伸长。内外稃异常导致灌浆后米粒变小、畸形,千粒重下降。该性状遗传稳定,受1对隐性基因控制,利用群体分离分析法(bulked segregation analysis,BSA)将AHL基因定位在第2染色体上的SSR标记RM14153与RM14167之间,遗传距离分别为1.19cM和1.34cM,物理距离为226kb。研究结果为AHL基因的图位克隆和功能研究奠定了基础。
Researches on rice flower development gene are very important in molecular breeding of rice for related traits. A rice flower mutant abnormal hull and lodicule (ahl) was identified from Jinhui 10 (Oryza sativa L. ssp. indica) treated by EMS. In ahl mutant, lemma and palea minished and significantly twisted along with the apparently elongated apical lodicules. This minished lemma and palea led to abnormal kernels after grain filling, meanwhile, the 1000-grain weight decreased. The trait was steadily inherited, and controlled by a pair of recessive gene. Using bulked segregation analysis method AHL was restricted between SSR markers RM14153 and RM14167, with genetic distances of 1.34 cM and 1.19 cM, respectively, which covered an approximate 226 kb region on the chromosome 2. The result is useful for further map-based cloning and functional analysis of AHL gene.