中文摘要：

本研究拟研究四甲基秋兰姆二硫化物（福美双）对肉鸡胫骨生长板超微结构的时序性影响。7日龄AA肉鸡饲喂含100mg·kg-1福美双的饲料48h，分别在8、9、13日龄剖杀试验鸡，迅速采取胫骨生长板放人2．5％戊二醛溶液于4℃固定，制备超薄切片，用透射电镜做超微结构观察。结果发现：8日龄鸡增殖软骨细胞变圆、坏死，线粒体增多、稍肿胀、出现致密颗粒，内质网断裂扩张呈泡状，而9日龄时更为严重；在8、9日龄时，前肥大软骨细胞多数无完整的细胞结构、细胞质溶解、有疑似凋亡细胞，13日龄时，前肥大软骨细胞已有完整的细胞结构，但细胞器仍呈坏死状、分泌旺盛。由此可见，福美双在短时间内可导致胫骨生长板增殖细胞、前肥大软骨细胞坏死，且前肥大软骨细胞更易受损，影响了软骨细胞的正常发育，推测其可能引发能量代谢障碍，并干扰了细胞内信号转导，使细胞周期紊乱，从而引发肉鸡胫骨软骨发育不良。

英文摘要：

The aim of this study was to study the ultrastructure of tibia growth plate in broiler chickens with a thiram （Tetramethylthiuram disulfide）-feeding procedure at the different stages. Seven-day-old AA broiler chickens were fed a regular diet containing thiram 100 mg · kg-lfor 48 h, then were dissected quickly, and growth plates were fixed in 2.5% glutaric dialdehyde solu- tion in 4 ℃ when they were 8, 9, 13 day-old. The ultrastructure of chondrocyte was observed by transmission electron microscope. The results showed that proliferative chondrocytes were slight-ly necrotic, increased numbers of swollen mitochondrion appeared electron-dense particles and ve- siculated cisternae of the endoplasmic reticulum were in rounding at 8 day old, and swollen mito- chondrion, breaked and dilated cisternae of the endoplasmic reticulum were more severe at 9 day- old; plasmolysis and doubtful apoptotic prehypertrophic chondrocytes were discovered at 8, 9 day-old, moreover, lighten necrotic prehypertrophic chondrocytes with complete cellular struc ture and increased secretory vacuoles with fine particles of proteoglycans emerged at 13 day-old. In conclusion, both proliferative cells and prehypertrophic chondrocytes of growth plates were damaged by thiram in a short time, especialy to prehypertrophic chondrocytes. We supposed that this changes would influence normal chondrocyte development. It was possible that thiram could disturb the energy metabolism and cellular signal transduction which confused the cell cycle, and resulted in tibial dischondroplasia.