中文摘要：

在 flowering 植物的 Microsporogenesis 在性复制起重要作用。在 EXINE FORMATION1 (DEX1 ) 的有缺陷者为在 Arabidopsis thaliana 的 exine 模式形成是必要的，这被报导了。然而，在调整小孢子开发的 DEX1 的功能大部分没被理解。在这研究，我们证明 DEX1 被在 situ 杂交使用 RNA 强烈在 tapetum 表示。dex1 小孢子从四个在版本以后被堕落并且流产。在四个的 callose 墙比在野类型在 dex1 异种是更薄的，建议 DEX1 影响在在花药开发期间的四个阶段的 callose 形成。RT-PCR 和即时 PCR 分析证明 CalS5，在小孢子开发期间在 callose 合成起一个重要作用，是极大地在 dex1 植物下面调整。DEX1 与一个 transmembrane 领域，一个细胞内部的领域和一个细胞外的领域编码膜蛋白质。一起，我们的结果证明 DEX1 为小孢子开发是必要的，可能由调整 CalS5 的表达式。

英文摘要：

Microsporogenesis in flowering plants plays important roles in sexual reproduction. It has been reported that DEFECTIVE IN EXINE FORMATION1 （DEX1） is essential for exine pattern formation in Arabidopsis thaliana. However, the functions of DEX1 in regulating microspore development are largely not understood. In this study, we show that DEX1 is strongly expressed in the tapetum by using RNA in situ hybridization, dexl microspores were degenerated and aborted after release from the tetrads. The callose wall in tetrads was thinner in the dexl mutant than in the wild type, suggesting that DEX1 affects callose formation at the tetrad stage during anther development. RT-PCR and real-time PCR analyses showed that CalS5, which plays an important role in callose synthesis during microspore development, was greatly down-regulated in dexl plants. DEX1 encodes a membrane protein with one transmembrane domain, one intracellular domain and one extracellular domain. Collectively, our results demonstrate that DEX1 is essential for microspore development, possibly by regulating the expression of CalS5.