中文摘要：

目的：研究且酞基因对肝癌细胞Huh7中基因表达的影响，为探讨且戳在肝癌发生发展中的分子机制提供依据。方法：以pcDNA3．1b质粒为载体，将HBx转入Huh7细胞中，同时将pcDNA3．1b作空白对照。用Western印迹法证实基因转染成功。应用Affymetrix公司的人类全基因组寡核苷酸基因芯片分析转染HBx基因前后，Huh7细胞的基因表达谱。随机选择转染前后有明显差异的6个基因（3个上调，3个下调），用荧光定量PCR技术进行验证。结果：从基因芯片结果分析中发现，与对照组相比,HBx转染后Huh7细胞中明显上调的基因有88个，下调的基因有111个，这些差异基因的功能涉及多个方面。结论：HBx基因对肝癌细胞的影响是双向的，既有转录激活的作用，同时又有转录抑制的功能。这些数据对于理解HBx基因在肝癌发生发展中的作用具有重要的参考意义。

英文摘要：

Objective： To study the effect of HBx on the gene expression profile in the hepatocellular carcinoma cell line Huh7 and to provide the molecular evidence for exploring the role of HBx in the tumorigenesis of hepatoma. Methods： HBx gene was transletted into Huh7 cells carried by pcDNA3.1 b plasmid which contained the entire open reading frame （ORF） of HBx gene. The empty vector of pcDNA3.1 b was used as a control. The HBx expression in transfected cells was confirmed by Western blotting as compared with those transfected with empty vectors. And then a microarray analysis was performed to detect the gene expression profile in Huh-7 cells by Human Genome U133 Plus 2.0 Array of Affymetrix-GeneChip. Six differentiated expressed genes （3 up-regulated and 3 downregulated） were selected randomly and verified with RFQ-PCR. Results： The microarray analysis revealed that 88 genes were significantly up-regulated and 111 genes were down-regulated in Huh7 cells after transfection with HBx gene as compared with those transfected with empty vector. The functions of these differentiated genes were related with many aspects. Conclusion： HBx affected hepatocellular carcinoma cell HUH-7 bi-directionally. It not only had transcription-activating effects but also had transcription-suppressing effects. These data had important value for understanding the role of HBx in tumorigenesis and development of hepatoma.