中文摘要：

以五年生人参根组织为试验材料,提取总RNA,反转录合成cDNA,利用RT-PCR法对人参鲨烯环氧酶（SQE）基因的cDNA进行克隆及序列分析,初步探讨人参皂苷生物合成途径中的影响因子。结果表明：获得人参SQE基因全长片段大小为1 636 bp,开放阅读框长1 611 bp,编码536个氨基酸残基,与其他植物核苷酸序列具有较高同源性,其中与三七、龙牙惚木、刺五加同源性分别为98%、96%、90%。

英文摘要：

Five-year ginseng root tissue was used as material. After extracting the total RNA and reversing transcription,Squalene Epoxidase（ SQE） gene was cloned and sequenced by RT- PCR technology. Then the impact factors of ginseng saponins＇ biosynthetic pathway was discussed. The results showed that Panax ginseng SQE was cloned successfully,with a full length of 1 636 bp,containing a 1 611 bp open reading frame that encode 536 amino acids. The nucleotide showed a high homology with other plants. Furthermore,phylogenic analysis on the sequence of SQE gene with other plants showed that P. ginseng was closely related to P. Notoginseng,Alalia elata and Eleutherococcus senticosus,and the identities of nucleotide sequences reached 98%,96% and 90%,respectively.