中文摘要：

目的：测定不同金属元素对拮抗链霉菌No.24菌株的生长及发酵产物活性的影响。方法：以枯草芽孢杆菌为供试菌株,采用管碟法测定添加不同金属元素后,No.24菌株发酵产物对枯草芽孢杆菌的活性变化情况。结果：生物活性测定结果表明,不同的金属元素对No.24菌株的生长及发酵产物的活性都有不同程度的影响。Hg^2＋、Ag^2＋和Pb^2＋能强烈抑制该菌的生长,Au^2＋、Sr^2＋、Co^2＋、Mo^2＋对其生长有一定的抑制作用,生长量略低于对照;Mn^2＋、Ba^2＋、Zn^2＋、Cu^2＋对其生长没有明显影响;Ca^2＋、Mg2＋对该菌的生长有一定的促进作用,菌体生物量明显高于对照。在0.001mol/L的浓度下,在培养基中加入Co^2＋、Au^2＋、Sr^2＋,其发酵产物的杀菌活性分别为对照的74.67%、55.10%和61.95%;Ag^2＋、Hg^2＋和Pb^2＋能强烈抑制该菌菌体生长;Ca^2＋和Mg^2＋则对菌的生长有明显的促进作用,当培养基中添加0.001mol/L、0.002mol/L的Mg^2＋时其发酵产物的杀菌活性分别提高到136.36%和154.55%;当培养基中添加0.001mol/L0、.002mol/L的Ca^2＋时其发酵产物的杀菌活性分别提高到122.73%和145.45%;在培养基中同时添加Mg^2＋和Ca^2＋时,且浓度为Mg^2＋0.002mol/L＋Ca^2＋0.003mol/L时,其发酵产物的对枯草芽孢杆菌的抑菌圈直径为40mm,与对照相比,其活性提高到了181.82%,生物量提高到了162.18%。

英文摘要：

In order to mensurate the effects of metal irons on the growth and bioaefivity of fermentation produets of No.24 strain. Methods： Take Bacillus subtills as object strain, different metal irons were added to No. 24 strain medium, by means of agar diffusion, bioaetivity of fermentation products was bioassayed. Results： Different metal irons have different effects on the growth of No.24. The results showed that Hg^2＋ , Ag^2 ＋ and Pb^2＋ had an intensive inhibition against the growth of Streptomyces spp. XiNong No. 24. Au^2＋ , Sr^2 ＋ , Co2^＋ had a degree inlfibition against the straln,and its biomass was lower than ek. Mn^2＋ ,Ba^2＋ ,Zn^2＋ ,Cu^2＋ had no effects on it. Ca^2＋ ,Mg^2＋ can stimulate the growth of the strain and biomass was higher than ck at the eonoentration of 0.001mol/L, when adding to Co^2＋ , Au^2＋ and Sr^2＋ , the fungieidal aetivity of its fermentation produets was only 74.67 %, 55.10%, 61.95 %, respectively in eomparision of the eontrol. When adding 0. 001mol/L Hg^＋2 , Ag^2＋ and Pb^2＋ to the mediums, the strain eould not synthesis the antibiotics, but Ca^2＋ , Mg^2＋ could inerease strongly the biomass of the fermentation products. When adding 0. 001 mol/L, 0.002mol/L Mg^2＋ to the medimns, the fungieidal aetivity of fermentation produets inereased by 136.36%, 154.55 %. When adding Ca^2＋ , it ean increased by 122.73 % and 145.45 %. When adding 0. 002mol/L Mg^2＋ and 0.003mol/L Ca^2＋ , the results showed that the diameter of inhibition zone inereased by 40mm,fungieidal activity and biomass inereased by 181.82% and 162.18%, respectively.