中文摘要：

目的探讨CD133、EGFRvⅢ在人脑胶质瘤组织中的共表达及临床意义。方法选择行手术治疗的脑胶质瘤患者40例，采用免疫组化双重染色法检测其脑胶质瘤组织中的CD133、EGFRvⅢ表达。结果40例患者脑胶质瘤组织中的CD133＋细胞、EGFRvⅢ＋细胞阳性表达率分别为100％、40％，二者双染阳性表达率为35％。CD133呈棕黄色，位于细胞质和细胞膜上，多数阳性细胞在肿瘤组织中散在分布，部分呈聚集现象；EGFRvⅢ呈深蓝色，主要分布在细胞膜上，细胞质中有少量分布；在肿瘤组织靠近血管区两种阳性细胞存在共聚集现象。结论CD133、EGFRvⅢ在脑胶质瘤组织中的分布具有相关性，在细胞水平上存在共表达，二者可以成为胶质瘤干细胞治疗的分子靶点。

英文摘要：

Objective To investigate the co-expression of CD133 and epidermal growth factor receptor variant m （ EGFRvⅢ ） in human glioma tissues and the clinical significance. Methods The expression of CD133 and EGFRv Ⅲ was determined by Double staining of immunohistochemistry in 40 surgical specimens of human glioma tissues. Results The positive rates of CD133 ＋ cells and EGFRvⅢ＋ cells in 40 cases were 100% and 40%, respectively. The double staining positive expression rate was 35%. CD133 was claybank in the cytoplasm and cell membrane, the majority of positive cells scattered in the tumor tissues, and some gathered together. EGFRv Ⅲ was dark blue, the mainly distribution was in the membrane, and a few were in the cytoplasm. There was co-aggregation of both positive cells in the tumor tissue near the vascular area. Conclusions There are correlations in the distribution and co-expression at the cellular level between CD133 and EGFRvⅢ in human glioma tissues. Both CD133 and EGFRvⅢ could become the molecule targets for glioma stem cell therapy.