采用免疫组织化学的卵白素-生物素-HRP复合物(ABC)法,以二氨基联苯胺(DAB)为呈色剂,观察高亲和性的神经营养物质受体(酪氨酸激酶受体),即TrkA,TrkB和TrkC在大鼠Scapa神经节(前庭神经节,VG)中的分布。结果显示,许多VG神经元分别对3种Trk受体呈阳性反应,受体位于神经元胞体。每一种Trk受体的反应强度在不同神经元间有区别,有弱、中等和强反应之分。对同一种Trk受体而言,阳性神经元的大小不等;统计结果显示:TrkA,TrkB和TrkC阳性神经元平均面积分别为330.8±7.6,303.89±10.6和355.05±8.3μm^2。该研究结果为Trk受体在VG内发挥维持神经元存活、保持其形态特征等的作用提供了形态学支持。
With the aim to examine the distribution of high-affinity neurotrophin receptors ( tyrosine kinase receptors, TrkA, TrkB and TrkC ) in the rat Scapa's ganglion (vestibular ganglion, VG), Avidin-Biotin-peroxidase Complex (ABC) method of immunohistochemistry with diaminobenzidine (DAB) as the chromogen to identify the immunoreactivities was employed in the present study. The results showed that many VG neurons were immunoreactive to each Trk isoform. The receptors were localized in the neuronal somata. The intensity of im- munoreactivity for each Trk receptor was different among neurons, ranging from weak, moderate to intense. For each individual Trk receptors, the labelled neurons were of different size ; the result sfatistical of analysis showed that the mean areas for neurons immunoreative to TrkA, B and C were 330.8 ± 7.6, 303.89 ± 10.6 and 355.05 ±8.3 μm^2 , respectively. The present study provids morphological substrate for the important roles played by Trk receptors in maintaining the survival and stabilizing the phenotype of VG neurons.