中文摘要：

【目的】通过对中华蜜蜂Apis cerana cerana嗅觉受体AcerOrco的表达及蛋白定位分析,阐明AcerOrco的表达特性,以期为进一步探索其功能提供理论依据。【方法】分别通过qRT-PCR技术和Western blot技术对中华蜜蜂气味受体AcerOrco mRNA及蛋白在内勤蜂和采集蜂5个组织部位（触角、头、胸、腹和足）中的相对表达量进行分析,采用免疫组化技术对AcerOrco蛋白的表达进行定位分析。【结果】定量结果显示,AcerOrco转录本在内勤蜂和采集蜂各组织中均有表达,其中触角中的表达量最高;该基因在采集蜂各组织中的表达量普遍高于内勤蜂。AcerOrco受体蛋白在内勤蜂和采集蜂各组织中也均有表达,在触角和头部的表达量明显高于其他组织。定位结果显示,在内勤蜂触角中,AcerOrco主要在毛形感器中表达,板形感器中表达不明显;在采集蜂触角的板形感器和毛形感器中均有表达,但毛形感器中的表达更多一些。【结论】获得了AcerOrco mRNA及其蛋白在内勤蜂和采集蜂各组织中的表达特性,并将这一蛋白表达定位于工蜂触角的毛形感器和板形感器中。

英文摘要：

【Aim 】 By the expression and localization analysis of olfactory receptor AcerOrco in the Chinese honeybee,Apis cerana cerana,we researched the expression characteristics of AcerOrco,in order to provide a theoretical basis for further exploring its function. 【Methods】Real-time PCR and Western blot technology were used respectively to determine the expression profiles of AcerOrco mRNA and protein in five tissues（ antennae,head,thorax,abdomen and legs） of nurse and forager bees. By immunohistochemical technique,cellular localization of the olfactory receptor in antenna was analyzed.【Results】The expression profiling showed that AcerOrco transcripts were detected in all the tested tissues of A. cerana cerana,and the most transcripts were found in antenna. The expression of this gene in different tissues of forager was generally higher than that in nurse. AcerOrco protein were also detected in all the tested tissues,and mainly expressed in antenna and head of nurse and forager. The location result showed that in nurse AcerOrco was detected in sensilla trihodea,but had no obvious expression in sensilla placodea. In forager AcerOrco was mainly expressed in sensilla placodea and sensilla trihodea,with higher expression level in sensilla trihodea. 【Conclusion】The expression profiles of AcerOrco at mRNA and protein levels were obtained,and the encoded protein was successfully localized in sensilla placodea and sensilla trihodea of antenna of worker bee.