中文摘要：

目的：探讨手术创伤引起的海马炎症反应对老年小鼠术后认知功能的影响。方法：96只雄性昆明小鼠随机分为对照组（NS组）、麻醉组（Ane组）、假手术组（Sham组）和手术组（Sur组）4组（n均为24）。使用Morris水迷宫进行认知功能测试。测试完毕后,采用Western blot和Real Time RT-PCR检测海马IL-1β、IL-6、TNF-α蛋白及mRNA表达水平。结果：术后第1、3、7天,4组动物目标象限停留时间百分比及1min内穿台次数差异有统计学意义（P均〈0.05）,且Sur组低于其他3组;4组动物海马IL-1β、IL-6、TNF-α蛋白及mRNA的表达差异有统计学意义（P均〈0.001）,Sur组较其他3组表达上调。结论：手术创伤能够引起老年小鼠术后认知功能障碍,其机制可能与术后海马组织中IL-1β、IL-6及TNF-α的表达上调有关。

英文摘要：

Aim：To investigate the effects of neuroinflammation in hippocampus triggered by surgical trauma on cognitive function in aged mice.Methods：A total of 96 male Kunming mice were randomly allocated into four groups（24 mice per group）：control group（group NS）,anesthesia group（group Ane）,sham group（group Sham） and surgery group（group Sur）.Cognitive function was assessed in Morris water maze.Then the hippocampus was immediately harvested for determination of the protein and mRNA expression of IL-1β,IL-6,and TNF-α by Western blot and Real Time RT-PCR.Results：On the 1st,3rd,and 7th day after treatment there were significant differences in the percent time spent in target quadrant and the number of platform-site crossovers within 1 min among the 4 groups（P 〈 0.05）,and the 2 parameters were lower in group Sur than the other 3 groups.On the 1st,3rd and 7th day,there were significant differences in protein and mRNA expression of IL-1β,IL-6,and TNF-α among the 4 groups（P 〈 0.001）,and the above parameters was upregulated in group Sur than the other 3 groups.Conclusion：Postoperative cognitive dysfunction induced by surgical trauma in aged mice may be associated with up-regulation of IL-1β,IL-6,TNF-α expression in hippocampus.