我们由联合刺激排放弄空(STED ) 显微镜和原子力量显微镜(AFM ) 造了一个综合成像系统。STED 显微镜基于 supercontinuum 纤维激光被构造, 42 nm 的一个超级侧面的决定被完成。与这个综合成像系统,词法特征,机械参数和荧光,超级分辨率成像同时被获得因为 nanobeads 和固定房间取样。这个新综合成像系统被期望在 nanobiology 和 nanomedicine 为研究在 nanoscale 获得全面信息。
We have built an integrated imaging system by combining stimulated emission depletion (STED) microscope and atomic force microscope (AFM). The STED microscope was constructed based on the supercontinuum fiber laser and a super lateral resolution of 42 nm was achieved. With this integrated imaging system, morphological features, mechanical parameters and fluorescence super resolution imaging were obtained simultaneously for both nanobeads and fixed cell samples. This new integrated imaging system is expected to obtain comprehensive information at the nanoscale for studies in nanobiology and nanomedicine.