中文摘要：

以凤丹白牡丹（Paeonia suffruticosa Andr.）继代愈伤组织为材料,研究不同外源H2O2浓度下牡丹愈伤组织生长过程中过氧化物酶（POD）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性的变化,探究酶活性变化与牡丹愈伤组织生长、分化的关系。结果表明,在愈伤组织生长过程中,当H2O2浓度达到50μmol/L以上时,愈伤组织形态均出现了不同程度的变化;100μmol/L H2O2处理的SOD活性在第5天达到峰值,与其他处理呈显著差异,之后逐渐下降;POD和CAT活性变化基本一致,在第5天出现明显上升,之后下降,然后趋于平稳。从形态观察和3种抗氧化酶活性结果来看,100μmol/L H2O2对牡丹胚性愈伤组织的形成和体细胞胚的诱导有促进作用。

英文摘要：

＂Fengdanbai＂ peony（Paeonia suffruticosa Andr.） was used to study, the changes of POD, CAT and SOD activity of peony during the callus growth process under different concentration exogenous H2O2. The relationship between the changes of enzymes activity and the growth and differentiation of callus was investigated. The results showed that in the process of the callus growth, the callus forms had different changes when the concentration of H2O2 was more than 50 μmol/L. The SOD activity of 100 μmol/L H2O2 treatment reached the peak in the 5th day with significant differences from other treatments, then declined. Under this treatment the changes of POD and CAT activities were basically same. It increased obviously and then declined, afterwards tended to be stable. The 100 μmol/L H2O2 promoted the embryonic callus formation of peony and the induction of somatic embryogenesis.