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中文摘要：
根据GenBank中细粒棘球绦虫EgA31序列（GenBank登录号为．AF067807）设计引物，以纽粒棘球绦虫mRNA为模板，RT-PCR扩增EgA31基因，将其克隆入pUCm-T载体，转化大肠埃希菌饵coli）DH5ct，筛选阳性克隆，经BamHI、SacI双酶切和PCR鉴定，获得阳性重组质粒pUCm—T／EgA31，并将测序正确的片段连接表达载体，成功构建重组质粒pET30a-Ega31。经序列分析和同源性比较，以及对其编码产物进行B细胞和T细胞表位分析，结果表明PCR扩增的特异条带为636bp，与预期相符，与GenBank已知基因序列同源性为100％。编码产物B细胞和T细胞联合表位预测，氨基酸区域可能在32～79、79～95、105～124和141～154位。
英文摘要：
Specific primers were designed and synthesized based on the reported EgA31 gene of Echirtococcus granulosus （GenBank Accession No. AF067807）. Total RNA was extracted from E. granulosus and its EgA31 gene was amplified by reverse transcription-polymerase chain raction （RT-PCR）. The PCR product was purified and cloned into plasmid pUCM-T, then transformed into Escherichia coli DHScc The recombinant plasmids were screened and identified by digestion with restriction enzyme and PCR amplification. The positive recombinant plasmid pUCM-T/EgA31 was confirmed by sequencing and homology comparison. Five parameters and methods were used to predict B-cell epitopes in amino acid sequence of EgA31. The amplified DNA fragment （636 bp） had an identity of 100% with the EgA31 gene sequence of E. granulosus. B-cell and T-cell epitopes of EgA31 were probably at or adjacent to 32-79, 79-95, 105-124 and 141-154 in its amino acid sequence.
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同项目期刊论文
The prediction of T- and B-combined epitope and tertiary structure of the Eg95 antigen of Echinococc
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