中文摘要：

目的了解髓系细胞触发受体1（TREM-1）基因重组慢病毒短发夹RNA（vshRNA）载体对脆弱拟杆菌脓毒症小鼠促炎因子表达及小鼠存活率的影响。方法（1）构建TREM-1 vshRNA载体。经实验确定脓毒症小鼠模型制作条件：腹腔注射2．5×10^9CFU／mL灭活脆弱拟杆菌0．5mL，刺激12h。（2）115只小鼠按随机数字表法分为健康对照组（3只）和脓毒症模型组、绿色荧光蛋白（GFP）干扰组、TREM-1干扰组、TREM-1半量干扰组。后4组每组28只，先分别经尾静脉注射等渗盐水、GFPvshRNA、TREM-1 vshRNA 2×10^8TU、TREM-1 vshRNA 1×10^8TU；1h后，将该4组小鼠制成脓毒症模型，健康对照组注射等体积等渗盐水。12h后处死5组小鼠（每组3只），取血检测血浆TNF-α、IL-1β和IL-6含量；取肝组织标本检测TREM-1 mRNA及其蛋白的表达水平。后4组小鼠每组所余25只用于观察腹腔注射后72h的存活率。（3）另建立脓毒症小鼠模型125只，其中100只分别于腹腔注射后1、2、4、6h尾静脉注射TREM-1 vshRNA 2×10^8Tu（每时相点25只），余下25只尾静脉注射等渗盐水为对照组。计算尾静脉注射后72h小鼠存活率。结果（1）与脓毒症模型组比较，TREM-1干扰组、TREM-1半量干扰组小鼠血浆TNF-α、IL-1β、IL-6含量均下降（P〈0．05），以TREM-1干扰组下降最明显。GFP干扰组中各促炎因子含量与脓毒症模型组接近（P〉0．05）。（2）与GFP干扰组比较，TREM-1干扰组及其半量干扰组小鼠肝组织TREM-1 mRNA表达均明显下调（P〈0．01），以TREM-1干扰组尤为明显（P〈0．05）。各组小鼠TREM-1蛋白表达情况与此一致。（3）脓毒症模型组、GFP干扰组小鼠腹腔注射后72h存活率均为16％。TREM-1干扰组及其半量干扰组分别为76％和44％（P〈0．05或P〈0．01）。（4）另建立的125只脓毒症小鼠模型中，对照组及腹腔注射后1、2、4、6h组其尾静脉注射后72h存活率分别为1

英文摘要：

Objective To investigate the effect of triggering receptor expressed on myeloid cells 1 （TREM-1） vshRNA vector on expression of inflammatory cytokines and survival rate in septic mice infected by Bacteroides fragilis. Methods （ 1 ） TREM-1 vshRNA vector was constructed. Bacteroides fragilis （2.5×10^9 CFU/mL, 0.5 mL） was intraperitoneally injected in each mouse, and septic model was reproduced after 12 hours. （2） One hundred and fifteen mice were divided into healthy control group （ n=3, HC）, sepsis group （ n = 28, S）, TREM-1 vshRNA group （ n=28, T）, TREM-1 vshRNA hd group（ n = 28, Th） , GFP group （n=28,G） according to random number table. Mice in S, T, Th, G groups were firstly injected with isotonic saline, TREM-1 vshRNA 2×10^8 TU, TREM-1 vshRNA 1×10^8 TU, GFP siRNA through tail vein, and then sepsis was induced after 1 hour. Mice in HC group were injected with equal volume of isotonic saline through tail vein. Three mice in each group were sacrificed after 12 hours for determination of plasma level of TNF-α, IL-1β and IL-6, and level of TREM-1 mRNA and its protein in hepatic tissue. The survival rate of other mice in each group was monitored for 72 hours. （3） In 125 mice sepsis was reproduced,among them 100 mice were injected with TREM-1 vshRNA 2×10^8 TU after 1, 2, 4, 6 hours through tail vein（25 mice at each time point） , other 25 mice were injected with equal volume of isotonic saline as control. The survival rate of mice in each group was recorded 72 hours after injection. Results （ 1 ） Com- pared with those in S group, the plasma level of TNF-α, IL-1β and IL-6 lowered in T and Th groups（ P 〈 0. 05 ） , especially in T group,while those in G group showed no obvious difference （ P 〉 0.05 ）. （2）Compared with those in G group, the level of TREM-lmRNA and its protein in hepatic tissue in T and Th groups decreased（ P 〈0. 01 ）, especially in T group. （3）The survival rate of mice in S and G group was 16% , which was obviously lower tha