中文摘要：

目的探讨血小板上表达的P-选择素对B16细胞分泌肿瘤坏死因子（tumor necrosis factor-α,TNF-α）和血管内皮生长因子（vascular endothelial growth factor,VEGF）的影响。方法将B16细胞分别与C57小鼠和P-选择素敲除小鼠的血小板共培养,用ELISA法检测上清中的肿瘤坏死因子和血管内皮生长因子的浓度。结果 B16细胞与C57小鼠的血小板共培养的血清中血管内皮生长因子的浓度明显高于与P-选择素敲除小鼠的血小板共培养的血清中的浓度,差异有统计学意义（P〈0.05）,而肿瘤坏死因子的浓度差异无统计学意义。结论血小板上表达的P-选择素促进了B16细胞分泌血管内皮生长因子,但对肿瘤坏死因子的分泌则没有影响。

英文摘要：

Objective To investigate the effects of platelet P - selectin on tumor necrosis factor - c~ and vascular endothelial growth factor secreted by B16 cells. Methods The B16 cells were co - cultured with the platelets from C57 mice and P - seleetin knockout mice. The TNF - a and VEGF concentrations in the supernatant were assessed using ELISA. Results The VEGF concentration was significantly higher in C57 group than that in P - selectin knockout group, though there was no significant difference in TNF - a. Conclusion Platelet P - selectin promotes B16 cells to secrete VEGF, but with no effect on TNF- a.