中文摘要：

根据植物细胞质雄性不育恢复基因编码的PPR（pentatricopeptide repeat）蛋白的特点,结合拟南芥PPR基因簇相似序列,扩增油菜野芥细胞质雄性不育（Nsa CMS）基因组DNA,筛选出一对简并引物,在Nsa不育系育性恢复后的可育材料和野芥亲本中可以同时扩增出符合预期的片段。片段长度为309bp,与萝卜CMS恢复基因核苷酸序列的一致性为69%,与拟南芥1号染色体臂上PPR基因簇核苷酸序列的一致性大于70%,与含波里马（Pol）CMS恢复基因的白菜型油菜相关序列一致性达85%。该片段编码的氨基酸序列含有两个相邻排列的PPR基序,可作为Nsa CMS育性恢复基因的候选片段。

英文摘要：

According to pentatricopeptide repeat（PPR） protein sequence encoded by restorer gene from cytoplasmic male sterile rapeseed plant,a pair of degenerate primers was obtained after PCR screening of Nsa CMS（hybrid of Brassica napus and Sinapis arvensis）.PCR results showed expected products specific to fertility restored plants and S.arvensis parent.The fragment was 309bp which was 69% identical to fertility restorer gene of Radish CMS,70% identical to PPR gene cluster from the first chromosome of Arabidopsis thaliana,and 85% identical to a B.rapa accession clone which restores the fertility of pol CMS.Further analysis revealed that the amino acid sequence encoded by this fragment contained two adjacent PPR motifs with 35 amino acids each.Results indicated this fragment could be part of the candidate Rf gene for Nsa CMS.