中文摘要：

本文探究了饥饿胁迫与饥饿后再投喂对虎斑乌贼幼体存活率、生长、行为、肝体比、摄食率以及消化酶活力的影响.在室内控制条件下开展了幼体（初始体质量为4.95±0.48 g）的饥饿（0、1、2、3、4、5、6 d）和再投喂（15 d）试验.结果表明：不同饥饿时间对虎斑乌贼的幼体存活率、体质量降低率、肝体比和消化酶活力影响显著.随着饥饿胁迫时间的增加,其存活率、肝体比呈下降趋势,其中饥饿3 d后,存活率开始明显下降,体质量降低率明显增大,幼体出现喷墨、互相残杀等异常行为;4种消化酶活力呈先下降后上升的趋势,淀粉酶活力以饥饿4 d组最低（0.07±0.02 U·mg-（-1）·prot-（-1））;脂肪酶活力以饥饿2 d组最低（18.47±2.07 U·g-（-1）·prot-（-1））,饥饿6 d组最高（57.60±3.98 U·g-（-1）·prot-（-1））,胃蛋白酶活力和胰蛋白酶活力以饥饿5d组（1.98±0.59 U·mg-（-1）·prot-（-1））和饥饿4 d（186.68±20.72 U·mg-（-1）·prot-（-1））最低.饥饿处理结束后,经15 d再投喂,各试验组存活率、特定生长率、肝体比和摄食率差异显著,幼体的存活率、特定生长率、肝体比和摄食率均与饥饿处理时间呈负相关;饥饿1和2 d组与对照组的存活率、特定生长率和肝肝体比无显著差异,而饥饿3-6 d组显著低于对照组;饥饿1-2 d组的摄食率明显高于对照组,而饥饿6 d组的摄食率明显小于对照组;各组淀粉酶与脂肪酶活力无显著差异,胃蛋白酶与胰蛋白酶活力差异显著,均以对照组最高（胃蛋白酶活力7.06±0.64U·mg-（-1）·prot-（-1）,胰蛋白酶活力914.67±26.54 U·mg-（-1）·prot-（-1））,饥饿6 d组最低（胃蛋白酶活力3.21±0.57 U·mg-（-1）·prot-（-1）,胰蛋白酶活力660.04±37.92 U·mg-（-1）·prot-（-1））.说明虎斑乌贼的幼体饥饿不可逆点（PNR）为第6天,且不能补偿生长.

英文摘要：

To investigate the effects of starvation and re-feeding on survival rate,behavior,growth and digestive enzyme activities of juvenile Sepia pharaonis,the manipulative laboratory experiments were conducted with 0- 6 days of starvation and 15 days of re-feeding to reveal the mechanism of compensatory growth. The results showed that starvation exerted a significant negative effect on the survival rate,growth,hepatosomatic index and digestive enzyme activities（ P0.05）. The survival rate and digestive enzyme activities declined during the whole starvation process. After 3 days,the survival rate began to decline significantly,the decrease rate of body mass increased obviously and juveniles experienced some abnormal behaviors,such as inkjet,fighting and so on. The effects of starvation on digestive enzyme activities of juveniles were significant,and the digestive enzyme activities declined and then rose during the whole starvation process. The lowest amylase activity occurred after 4 days of starvation（ 0.07±0.02 U·mg-（-1）·prot-（-1））. The lowest lipase activity occurredafter 2 days of starvation（ 18.47±2.07 U·g-（-1）·prot-（-1））,and the highest after 6 days（ 57.60±3.98 U · g-（-1）· prot-（-1））. The lowest pepsin and trypsin activities occurred after 5 days（ 1. 98 ± 0. 59U·mg-（-1）·prot-（-1）） and 4 days（ 186.68±20.72 U·mg-（-1）·prot-（-1）） of starvation,respectively. The effects of re-feeding on survival rate,growth,hepatosomatic index and digestive enzyme activities of juveniles were significant. The survival rate,specific growth rate,hepatosomatic index and feeding rate were negatively correlated with hunger processing duration. The survival rate,specific growth rate and hepatosomatic index showed no significant difference between 1 day starvation group and 2days starvation group（ P0.05）. The survival rate,specific growth rate and hepatosomatic index of3 days to 6 days starvation groups were significantly lower than the control group. The feedin