中文摘要：

用RT—PCR的方法从重要的高原作物青稞（Hordeum vulgare L．var．nudum Hook.f.）中克隆到了脱水素基因dhn4，其编码区长为678bp，所编码的蛋白质DHN4为YSK7型脱水素、经预测，该蛋白二级结构易形成α-螺旋，并无固定的三级结构．将dhn4基因cDNA编码区定向克隆到载体PET30-c上，获得了重组质粒PET—dhn4，该重组质粒再转化到大肠杆菌BL21菌株，37℃经1.5mmol／LIPTG诱导获得了大量分子量约为35kD的融合蛋白,然后用亲和层析的方法对该蛋白进行纯化，得到了纯化的脱水素DHN4蛋白．

英文摘要：

A dehydrin gene （dhn4） cDNA fragment has been obtained via RT-PCR from Tibetan hulless barley（Hordeum vulgate L. var. nudum Hook. f. ）. It indicated that dhn4 encoded a YSK2 type dehydrin （DHN4）. The secondary structure of DHN4 predicated with software Anthepro 5.0 is prone to α-helix, and the tertiary structure predicated by SWISS-PROT indicated intrinsically unstructured. The coding region of the dhn4 cDNA recombinated into PET30-c vector was transformed into the Eschrichia coli overexpression strain BL21, and the protein DHN4 was then produced. The fused 35 kD proteins induced with 1.5 mmol/L IPTG at 37 ℃ for 4 hours were overexpressed and then purified by Ni-NTA Purification method. The properties were visualized by SDS-PAGE and identified by Western-blotting analysis. These experiments described above made advantage of the following researches on the physiological and biochemical functions of dehydrins.