中文摘要：

目的探讨缺氧诱导因子1α（HIF-1α）在胃癌组织中的表达和细胞定位以及其与临床病理和临床预后的关系。方法收集2015年7至9月安徽医科大学第-附属医院普外八病区胃癌患者的27对新鲜配对的胃癌组织及癌旁正常组织，采用实时荧光定量PCR（qRT-PCR）和Western印迹方法检测胃癌组织和癌旁正常组织中HIF-1αmRNA和蛋白的表达水平。收集2006年12月至2008年9月安徽医科大学第-附属医院普外科胃癌患者的191份胃癌组织和46份单纯随机抽样法抽取的癌旁正常组织，采用免疫组化方法检测胃癌组织和癌旁正常组织中HIF-1α蛋白的表达及描述微血管密度（MVD）计数的CD34的表达，并且分析HIF-1α在胃癌组织中胞质、胞核的不同定位与MVD、临床病理和临床预后之间的关系。结果PCR定量结果显示，HIF-1αmRNA在胃癌组织中的相对表达量高于癌旁正常组织（0．625±0．170比0．218±0．036，t=2．336，P=0．023）。Western印迹结果显示，HIF-1α蛋白在胃癌组织中的表达强度明显高于癌旁正常组织。免疫组化结果显示，胃癌组织HIF-1d阳性率显著高于癌旁正常组织（67．54％比45．65％，P=0．006）；其中，胃癌组织中的胞质定位阳性率与癌旁正常组织比较差异无统计学意义（35．08％比45．65％，P=0．138）；胞核定位阳性高于癌旁正常组织（37．17％比2．17％，P〈0．001）。组织高分化和TNM临床早期（Ⅰ＋Ⅱ期）患者胃癌组织中胞质HIF-1α的表达阳性率显著高于组织低分化患者（P=0．008）和TNM临床中晚期（Ⅲ＋Ⅳ期）患者（P=0．019）；组织低分化患者胃癌组织中胞核HIF-1α的表达阳性率显著高于组织高分化患者（P=0．043）。胃癌组织中胞核HIF-1α阳性者的MVD平均数（115．6±7．8）显著高于胞质HIF-1α阳性者（93．1±7．5，t=2．077，P=0．040）。中位随访56个月（3—81个月），Kaplan—Meier生存分析L

英文摘要：

Objective To explore the expression level and location of hypoxia-inducible factor 1 α （HIF-1 α） in gastric cancer （GC） tissues and their relationship with elinicopathological features and clinical outcomes. Methods From July to September 2015,27 pairs of fresh paired GC tissues and adjacent normal tissues were gathered from the Eighth Department of General Surgery of the First Affiliated Hospital of Anhui Medical University. Quantitative real-time PCR （qRT-PCR） and Western blot were performed to detect the expression of HIF-1α mRNA and protein in these tissues. A total of 191 GC tissues and 46 randomly selected adiacent normal gastric tissues were consecutively collected between December 2006 and September 2008from Department of General Surgery of the same hospital. Immunohistochemistry were performed on them to detect the expression of HIF-1α and CD34 [ described in terms of microvessel density （MVD）], and correlation of different locations of HIF-lct （in cytoplasm or nucleus） with MVD, clinieopathological features, and clinical prognosis was analyzed. Results The average relative expression level of HIF-1α mRNA in GC tissues （0. 625 ± 0. 170） was significantly higher than in normal adjacent tissues （0. 218 ± 0. 036, t = 2. 336,P = 0. 023） by qRT-PCR. From the results of Western blot, the expression level of HIF- lct protein increased in GC tissues compared with its corresponding normal tissues. Immunohistochemistry results revealed that positive HIF-1α staining was observed in 67.54% GC tissues and 45.65% normal tissues, with significant difference （P =0. 006）. And 35.08% in GC and 45.65% in normal tissues were cytoplasmic positive （P=0. 138） ; while 37. 17% in GC and only 2. 17% in normal tissues were nuclear positive, with significant difference （P 〈 0. 001 ）. High differentiation group and TNM clinical early stage （ Ⅰ ± Ⅱ ） group had significantly higher cytoplasmic HIF-lot expression positive rate compared with low differentiation group （ P = 0.