中文摘要：

目的：应用RNA干扰技术抑制NOB1基因的表达，并在卵巢癌HEY细胞上观察NOB1下调对细胞增殖能力和细胞周期的影响。方法：NOB1特异性siRNA构建于慢病毒载体，感染卵巢癌HEY细胞后，Real—timePCR和Western验证NOB1的抑制效率，MTT、Brdu实验观察NOB1基因沉默对HEY细胞增殖的作用，PI染色流式细胞仪检测细胞周期变化。结果：shRNA慢病毒感染HEY细胞能够将NOB1基因mRNA表达抑制82．2％，蛋白表达也显著降低，MTT和Brdu实验显示细胞增殖水平显著变弱，流式细胞仪检测证实细胞出现G1期阻滞。结论：应用RNA干扰技术能在HEY细胞上有效沉默NOB1基因，并降低卵巢癌细胞的增殖能力，使细胞阻滞在G1期。

英文摘要：

Objective： To down- regulate NOB1 by RNA interference, and investigate its effect on proliferation and cell cycle of ovarian cancer cell line HEY. Methods： Small interference RNA （siRNA） targeting NOB1 gene was cloned into lentivirus vector. Then, siRNA - NOB1 lentivirus particles were infected into HEY cells in order to silence the expression of NOB1. MTT assay, BrdU cell prolifera- tion assay and flow cytometry were performed to detect the proliferation and cell cycle on HEY. Results： NOB1 gene could be down - regulated at both mRNA （ ↓ 82. 2% ） and protein level. Results from MTT assay, BrdU cell proliferation assay and flow cytometry showed that siRNA - NOB1 - lentivirus inhibited the proliferation of HEY cells with statistically significant difference. At the same time, HEY cells were arrested in G1 phase by NOB1 silence. Conclusion： Silencing NOB1 gene by RNA interference can inhibit the proliferation of HEY cells and arrest the cell cycle in G1 phase.