以石漠化地区造林树种青冈栎幼苗为试验材料,在盆栽条件下,分别用内生菌根真菌摩西球囊霉(Glomus mosseae,Gm)、根内球囊霉(Glomus intraradices,Gi)、外生菌根真菌彩色豆马勃(Pisolithus tinctorius,Pt)和3种菌根真菌混合菌剂进行接种,利用根系扫描仪Epson Perfection V700和根系分析软件WinRhizo,研究了正常水分、中度干旱和重度干旱条件下菌根真菌对青冈栎幼苗根系形态的影响。结果表明:在土壤干旱条件下,接种菌根真菌使青冈栎根系生物量、总根长、根表面积和根体积显著增加,使根平均直径减小;在土壤中度干旱胁迫下,Pt处理根表面积显著高于Gm和Gi处理,而根平均直径显著小于Gm和Gi;在重度干旱胁迫下,Pt处理根生物量和根体积显著小于Gm和Gi,根平均直径显著大于Gm和Gi;相关性分析表明,菌根侵染率与根生物量和总根长呈极显著正相关。本研究表明,在干旱条件下,菌根真菌可改变青冈栎幼苗根系形态,促进碳水化合物向根系积累和分配。
Cyclobalanopsis glauca is a common afforestation species in the karst rocky desertified region of Southwest China. We used two arbuscular mycorrhizal fungi Glomus mosseae (Gm) and Glomus intraradices (Gi), an ectomycorrhizal fungus Pisolithus tinctorius (Pt), and mixed mycorrhizal fungi to inoculate C. glauca seedlings. The seedlings were maintained under three soilwater conditions: well watered, moderate drought, and serious drought. Then, the root scanner Epson Perfection V700 and root analysis software WinRhizo were used to measure root morphological indexes, in order to study the effects of mycorrhizae and drought stress on the C. glauca seedlings. The results showed that under the two conditions of drought stress, root biomass, total root length, root surface area, and root volume of the inoculated seedlings were significantly improved, while average root diameter decreased significantly. Under moderate drought stress, root surface area of the seedlings treated with Pt was significantly higher than that of the seedlings treated with Gm or Gi, while the average root diameter of the seedlings treated with Pt was smaller than that of the seedlings treated with Gm or Gi. However, under severe drought stress, root biomass and root volume of the seedlings treated with Pt were significantly lower than those of the seedlings treated with Gm or Gi, while average root diameter of the seedlings treated with Pt was significantly larger than that of the seedlings treated with Gm or Gi. The correlation of mycorrhizal infection rate with root biomass and total root length was positive. This experiment suggests that the root morphology of C. glauca seedlings was changed by the mycorrhizal fungi, which promoted the accumulation and allocation of carbohydrates to the roots of C. glauca under conditions of drought stress.