中文摘要：

用荧光光谱法、分光光度法研究了水溶液中曙红（TBF）与牛血清白蛋白（BSA）的相互结合反应．研究表明BSA与TBF的结合数为n=1.266．其平衡常数KA=2．05×10^7L／mol．根据Forster非辐射能量转移理论，求算了给体（BSA）受体（TBF）间的距离r=2．06nm和能量转移效率E=0．76．实验表明：曙红与牛血清白蛋白相互结合造成的荧光猝灭为单一的荧光静态猝灭过程．

英文摘要：

The binding reaction between Tetrabromofluorescein （TBF） and bovine serum albumins （BSA） in aqueous solution was studied by fluorescence and ultraviolet-visible abosorption spectra. The results indicates that the binding sites is 1. 266 and the equilibrium constant KA is 2. 05 × 10^7 L/mol. The SternVolmer curve on relationship, which the fluorescence of BSA quenched by illustrates the combination reaction of series of concentrations shows good linear TBF and BSA is a single static quenching progress. The shortest binding distance （r= 2.06 nm）and energy transfer efficiency（E= 0. 76） between donor（BSA） and acceptor（TBF） were obtained by Forster＇s nonradiative energy transfer mechanism.