成功建立了高效降解敌敌畏的海藻酸钙交联固定木霉AMT-28分生孢子体系。与固定化的菌丝或自由悬浮的菌丝相比,固定不同浓度分生孢子小球的敌敌畏降解能力均有显著提高,其中以每100 mL海藻酸钠-硅藻土悬液固定107个分生孢子小球的效果最佳。同时,孢子固定化小球在敌敌畏降解过程中保持了较高的储存稳定性和良好的重复使用性能。因此,此项技术必将在敌敌畏水污染处理方面得到广泛的应用。
An immobilizing conidia approach was used to study the degradation ability of dichlorvos in Trichoderma atroviride T-DNA insertional mutant AMT-28. The beads immobilized 107 conidia per 100 mL of Naalginate and diatomite solution exhibited the highest degradation abilities. The immobilized conidia showed enhanced degradation abilities compared with immobilized or freely suspended mycelia. The immobilized cells were also kept good storage capacity and reusability. Therefore, the immobilization of AMT-28 conidia technique will be widely used in biodegradation for DDVP in aqueous solution.