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中文摘要：
目的筛选抗失巢凋亡食管癌细胞亚系，研究抗失巢凋亡与癌细胞转移间的关系。方法采用流式细胞技术检测食管癌细胞系EC9706、KYSE30、YES2和NEC无基质凋亡情况，用Poly-HEMA包被培养板对NEC细胞进行四轮抗失巢凋亡筛选，获得抗失巢凋亡细胞亚系NEDP4。将上述各细胞系经尾静脉接种BALB／c-nu裸鼠，观察各细胞系发生肺转移的情况。结果凋亡频率低的EC9706和KYSE30发生肺转移的能力分别为100％和83％，肺转移灶为121和83。易凋亡的YES2和NEC细胞肺转移灶为9和7，无明显肉眼转移灶。NEGP4细胞其发生凋亡的比例由亲本细胞的73．14％减少到18．14%，并可见肉眼转移灶，镜下转移灶是亲本细胞的5倍。结论食管癌细胞抗失巢凋亡与其肺转移能力高度相关，应用抗失巢凋亡筛选可获得高转移能力食管癌细胞。
英文摘要：
Objective To establish an anoikisresistant subpopulation of cell lines and to observe the relationship between metastasis and anoikis resistance in esophageal carcinoma cells. Methods Analyze the anoikis-resistant ability of EC9706, KYSE30, YES2 and NEC with Flow cytometry. The cells were cultured in the plates coated with Poly HEMA to select the anoikis-resistant cells through four rounds. The sub-line NEC-P4 were filtrated from the NEC parent cell. The BALB/c nu Mice were intravenously injected with half million cells per animal for NEC-P4 and examined for metastases development. Results The ability of metastasis of the four cells showed that anoikis-resistant EC9706 and KYSE30 had obvious lung metastasis. Visible lung metastasis of EC9706 was 100% and KYSE30 was 83%. EC9706 had 121metastasis sites per lung and KYSE30 had 83. Anoikis-sensitive YES2 and NEC had only 9 and 7. There were no visible lung metastasis in YES2 and NEC guoup. The anoikis-resistant cells we called NEC-P4 were selected through 4 rounds from the anioikis-NEC parent cells. The apoptosis of NEC-P4 was about 18% and NEC parent was about 78% when cultured in plates coated with poly-HEMA. NEC- P4 group had visible lung metastasis and 5 times of HE metastasis sites per lung in average than NEC group. Conclusion There were close relationship between metastasis and Anoikis-resistance in esophageal carcinoma cells. The anoikis resistant cells selected from parent cells always had high metastasis ability.
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