中文摘要：

目的：探讨肝素联合肝干细胞（WB-F344细胞）经脾移植对大鼠急性肝损伤的治疗作用。方法：构建携带绿色荧光蛋白（GFP）基因的慢病毒载体,体外培养、扩增WB-F344细胞,携带GFP基因的慢病毒转染WB-F344细胞。通过腹腔注射四氯化碳（CC l4）建立大鼠急性肝损伤模型,造模后24 h分别将1 mL含2×107个肝干细胞和8μL肝素的悬液经脾注入大鼠体内。移植术后1 d获取肝脏和脾脏标本进行荧光检测,分别于术后3,7,14和28 d尾静脉抽血送肝功能检测,获取肝脏组织行病理学检测。结果：经慢病毒转染3 d后,WB-F344细胞表达GFP;GFP阳性细胞经脾移植1 d后到达肝脏;实验组大鼠的肝功能和肝脏损伤修复明显优于对照组（P〈0.05）。结论：经脾联合肝素和WB-F344细胞移植对CC l4所导致的大鼠急性肝损伤有明显的治疗作用。

英文摘要：

Objective To determine the treatment effects of transplanted hepatic progenitor cells（WB-F344 cells） combined with heparin on the acute liver injury in SD rats.Methods A total of 2×107 hepatic stem cells（WB-F344） infected with GFP lentivirus and 8 μL heparin were transplanted through the spleen in SD rats with acute liver injury,which was induced by an intraperitoneal injection of CCl4.The liver and spleen tissues underwent fluorescence examination 1 day after the transplantation.The liver functions were tested,and the liver tissues were histopathologically examined on the 3rd,7th,14th,and 28th day of the cell transplantation.Results The transfected WB-F344 cells expressed GFP 3 days after the lentivirus infection and were found in the rat liver 1 day after the WB-F344 transplantation.The liver function and histopathological recovery of the liver tissues in the group of WB-F344 transplantation were better than those of the control group（P0.05）.Conclusion Transplantation of hepatic stem cells combined with heparin can promote the liver recovery in rats with acute liver injury induced by CCl4.