欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
DEPDC1（DEP domain containing 1）是一个新的肿瘤相关基因,在多种恶性肿瘤的发生发展进程中起着重要作用。我们前期工作中在鼻咽癌细胞内沉默了DEPDC1的表达,发现抑制细胞增殖并诱发细胞凋亡。本研究旨在探讨沉默DEPDC1表达后,对鼻咽癌细胞HNE-1和CNE-1侵袭迁移能力的影响及其分子机制。结果显示,siRNA介导DEPDC1表达沉默后,细胞侧向运动能力、侵袭及迁移能力显著降低。qRT-PCR及Western印迹检测发现DEPDC1沉默导致EMT上游关键转录因子Twist1及间质细胞标志分子Vimentin表达显著下调。这些研究表明,鼻咽癌细胞中DEPDC1通过调节Twist1等EMT关键分子的表达在细胞侵袭转移过程中起关键作用。推测DEPDC1在鼻咽癌中高表达可能对于促进其侵袭转移具有重要作用,进而促进肿瘤发生发展,但具体分子机制仍有待更深入研究。
英文摘要：
DEPDC1（ DEP domain containing 1） is a novel tumor-related gene and plays important roles in the development of a variety of malignant tumors. Study indicated that DEPDC1-depletion repressed cell proliferation and induced apoptosis in HNE-1 nasopharyngeal carcinoma cells. This study aims to explore its role in cell migration and invasion. The results demonstrated that DEPDC1 depletion significantly inhibited the migration and invasion in HNE-1 and CNE-1 cells by wound healing and Trans Well assays. The expression of Twist1 and Vimentin were down-regulated as determined by qRTPCR and Western blot. It suggested that high expression of DEPDC1 enhanced the migration and invasion in nasopharyngeal carcinoma,and the underlining molecular mechanism needed to be elucidated.
同期刊论文项目
同项目期刊论文
期刊信息
位置：