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突触后致密体蛋白95在大鼠出生后前额叶皮质的发育

ISSN号：1007-6611
期刊名称：《山西医科大学学报》
时间：0
分类：R338.1[医药卫生—人体生理学;医药卫生—基础医学]
作者机构：[1]天津中医药大学中西医结合室,天津300193, [2]武警后勤学院生理与病理学教研室, [3]武警后勤学院卫生教研室, [4]武警后勤学院附属医院
相关基金：国家自然科学基金资助项目（81271224）; 武警后勤学院创新团队基金资助项目（WHTD 201308）

关键词：突触后致密体蛋白95(PSD-95), 前额叶皮质, NMDA受体, 发育, postsynaptic density protein-95, prefrontal cortex, NMDA receptor, development

中文摘要：

目的探讨突触后致密体蛋白95（postsynaptic density protein 95,PSD-95）在大鼠前额叶皮质发育中的表达变化。方法采用real-time PCR和Western blot分别测定出生后第1,7,14,21,30,50天的Wistar大鼠前额叶皮质PSD-95的mRNA及蛋白水平的表达。结果大鼠在出生后不同时间,其前额叶皮质PSD-95 mRNA及其蛋白表达量有一定的特点。在出生后第1-7天,PSD-95 mRNA及其蛋白表达水平均较弱;出生后第14-30天,PSD-95 mRNA及其蛋白表达水平明显增强（P〈0.01）;出生后第50天继续表达在较高水平（P〈0.05）,但较出生后第14-30天已有所减弱。结论 PSD-95在前额叶皮质中的表达变化具有时间特异性,此模式可能与突触的发育及成熟变化密切相关。

英文摘要：

Objective To investigate the changes of expression of postsynaptic density protein-95 （ PSD-95 ） in the prefrontal cortex of rat at postnatal stage. Methods Real-time PCR and Western blot were used to detect the expression of PSD-95 mRNA and protein respectively at postnatal day 1, 7, 14, 21, 30 and 50. Results The expression of PSD-95 mRNA and protein in prefrontal cortex showed some characteristics during the postnatal development. At postnatal day 1 -7 ,the expression levels of PSD-95 mRNA and protein were weak. At day 14 -30 ,the expression levels of PSD-95 mRNA and protein were significantly increased compared with that at postnatal day 1 （P 〈 0.01 ）. At postnatal day 50, the expression levels of PSD-95 mRNA and protein were expressed in a high level（ P 〈 0.05 ）, but they were weaker than those at postnatal day 14 - 30. Conclusion The expression of PSD-95 shows a special temporal distribution pattern, and PSD-95 may participate in the development and maturation of synapse.