中文摘要：

建立了一种置换型荧光偏振免疫分析方法（DFPIA）检测水样中除草剂丁草胺。研究了不同结构示踪物对丁草胺DFPIA灵敏度的影响，优化筛选出一种荧光示踪物抗体组成的免疫复合物；考察了DFPIA置换反应动力学。所建立的DFPIA法特异性良好，检卅限为22．6μg／L，半数抑制浓度（IC50）为321．3μg/L，检测范围为60～1472mg／L，检测时间为12．5rain，添加回收率在88．1％～112．5％间，免疫复合物可以在4℃下稳定保存至少1星期以上。本方法适于环境水样中丁草胺的筛选检测。

英文摘要：

A displacement fluorescence polarization immunoassay （DFPIA） was developed for the detection of herbicide butachlor. The influence of the tracer structure on the assay performance was examined and an immunocomplex of tracer with longest arm was screened. The displacement reaction curves showed a kinetic nature of second-order reaction. The limit of detection （LOD） was 22.6 μg/L with 321.3 μg/L of IC50 and 59.6-1472.1 μg/L of working range. The detection time was only 12.5 min, and the recovery ranged from 88.1% to 112.5%. The immunocomplex was stable for more than one week at 4 ℃. The proposed DFPIA was suitable as a surveillance screening method for the detection of the herbicide butachlor in environmental water samples.