中文摘要：

随着分子生物学和标记技术的进步，光学成像和分析有了很快的发展，无论是从理论上还是应用上都有所突破，展现了良好的前景。本文综述了近年来在分子生物探测中光子学的情况，简介了几种突破光学衍射极限的探测方法，着重阐明了与生化技术密切相关的荧光共振能量转移和荧光相关光谱显微术及其在分子生物学中的应用。

英文摘要：

Whether on theory or in applications, optical imaging and analysis have gained great progresses with the developments of biological studies and labeling techniques and which exhibits a good prospect. Recently, the biophotonics advances and several new ways of breaking diffraction limit on the optical imaging are described in this paper. In particular, the progresses on the fluorescence resonance energy transfer microscopy and fluorescence correlation spectroscopy, as well as their applications for resolving molecular biologic problem are discussed in detail.