位置:成果数据库 > 期刊 > 期刊详情页
水稻内生放线菌OsiRt-1的分离鉴定及对稻瘟病的防治作用
  • ISSN号:0253-2654
  • 期刊名称:《微生物学通报》
  • 时间:0
  • 分类:S964.2[农业科学—水产养殖;农业科学—水产科学]
  • 作者机构:[1]湖南大学生物学院植物功能基因组学与发育调控湖南省重点实验室,湖南长沙410082, [2]湖南亚华种业科学研究院,湖南长沙410119
  • 相关基金:国家自然科学基金项目(No.51378191,31571635); 湖南省科技计划项目(No.2014WK2005)
中文摘要:

【目的】从水稻中分离、筛选并鉴定出对稻瘟病真菌具有拮抗性能的内生放线菌,对高抗菌株进行稻瘟病生防效果评定,初步探讨其作用机制。【方法】采用4种分离培养基对水稻内生放线菌进行分离,用平板对峙法筛选出对稻瘟病菌拮抗性能最好的菌株,结合其菌落形态、生理生化及16S rRNA基因序列分析进行鉴定;用环境扫描电镜(SEM)观察拮抗菌对稻瘟病菌菌丝形态影响,用菌丝生长速率法对拮抗菌发酵滤液进行抗菌活性评价;大田条件下,喷洒拮抗菌孢子液于水稻,检测其稻瘟病控制效果;同时,对拮抗菌进行产酶和次级代谢产物分析,检测其聚酮合酶基因(PKSⅡ型)和非核糖体多肽合成酶基因(NRPS)。【结果】从1 800个水稻样品中分离出117株内生放线菌,从中筛选出拮抗性能最好的菌株Osi Rt-1,鉴定为米修链霉菌Streptomyces misionensis。该菌株使稻瘟病菌菌丝出现畸形,其无菌滤液对病菌菌丝生长的抑制率为28.06%;大田条件下,Osi Rt-1对水稻苗瘟、穗瘟均有较好防效,其中对苗瘟防效为7.76%,穗瘟防效高达25.65%,损失率降低了17.46%。与之对应,Osi Rt-1处理过的水稻地上部分,Osi Rt-1所占内生放线菌的比例明显高于未处理水稻。该菌株具有可能降解真菌细胞壁的纤维素酶、蛋白酶活性,同时可产生植物生长促进剂铁载体、IAA、ACC脱氨酶。菌株Osi Rt-1呈现PKSⅡ型和NRPS阳性。【结论】菌株Osi Rt-1是一株具生防潜力的内生放线菌,在农业上具有实际研发价值。

英文摘要:

[Objective] To isolate, screen and identify rice endophytic actinomyces with antagonistic activity against rice blast pathogen, to evaluate the disease control effect of the selected antagonist and to study its potential antagonistic mechanism. ]Methods] We used 4 isolation media to isolate endophytic actinomycetes from surface sterilized rice. Using dual culture assay, we screened the strain with antagonistic activity and identified it by morphological, physiological and biochemical characteristics and 16S rRNA gene sequence analysis. We observed the effect of the antagonist on the hyphae of pathogen under scanning electron microscopy and tested the in vitro antagonistic activity of fermentation broth by growth rate method. In field condition, we evaluated the disease control efficiency after spraying the antagonist spore solution on rice. Meanwhile, we analyzed enzymes and secondary metabolites production of the antagonist, and amplified its polyketide synthases (PKS II ) and nonribosomal peptide synthetases (NRPS) genes. ]Results] From 1 800 rice samples, a total of 117 endophytic actinomycetes were isolated, among which, OsiRt-1 exhibited remarkable antagonistic activity. OsiRt-1 was identified as Streptomyces misionensis. The pathogen Magnaporthe oryzae treated by OsiRt-1 revealed significant morphological alterations in hyphe and its mycelial growth was inhibited by culture filtrates of OsiRt-1 with a percent of 28.06%. In field condition, OsiRt-1 spore solution could reduce seedling blast severity by 7.76%, panicle blast disease severity by 25.65% and lose rate by 17.46%. The separation rate of OsiRt-1 from above-ground portion of rice treated with OsiRt-1 is higher than the not treated ones. Besides, OsiRt-1 is positive for PKS I[ and NRPS genes and can produce cellulase, protease, siderophore, IAA and ACC deaminase. ]Conclusion] Endophytic Streptomyces misionensis OsiRt-1 has the application potential in agriculture as a biocontrol agent against rice blast disease.

同期刊论文项目
同项目期刊论文
期刊信息
  • 《微生物学通报》
  • 中国科技核心期刊
  • 主管单位:中国科学院
  • 主办单位:中国科学院微生物研究所 中国微生物学会
  • 主编:赫荣乔
  • 地址:北京市朝阳区北辰西路一号院3号中国科学院微生物研究所内B401室
  • 邮编:100101
  • 邮箱:xuj@sun.im.ac.cn
  • 电话:010-64807511
  • 国际标准刊号:ISSN:0253-2654
  • 国内统一刊号:ISSN:11-1996/Q
  • 邮发代号:2-817
  • 获奖情况:
  • 1992年中国科学技术协会优秀学术期刊三等奖,1992年国家科委中共中央宣传部新闻出版署“全国优...,2000年中国科学院优秀期刊三等奖,中国期刊方阵“双效”期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),波兰哥白尼索引,美国剑桥科学文摘,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:29487