中文摘要：

目的 探讨通过＂自组装＂（Self-Assembly）培养技术,以生长分化因子-5（GDF-5）诱导人骨髓间充质干细胞（hMSCs）,分化形成软骨组织的可能性及效果.方法 将第3代hMSCs用含GDF-5的软骨诱导液定向诱导培养.3周后重悬细胞,自组装培养.对自组装组织团块经行大体观察、逆转录-聚合酶链反应（RT-PCR）、免疫组织化学、软骨相关染色检测.结果 自组装组织团块有类似于软骨的外观,Ⅱ型胶原mRNA表达明显,组织学显示Ⅱ型胶原和蛋白多糖表达阳性.GDF-5诱导组Ⅱ型胶原免疫组织化学平均吸光度为（0.1678±0.0222）,对照组平均吸光度为（0.0908±0.0145）,差异有统计学意义（P＜0.05）.结论 自组装法培养hMSCs能形成具有软骨分子生物学、组织学和生物力学特性的组织团块,而GDF-5能够增强此过程中细胞的软骨表达.

英文摘要：

Objective To investigate whether cartilage tissue can be developed by self-assembling process with chondrogenically differentiated human mesenchymal stem cells （hMSCs）. Methods hMSCs were cultured and differentiated chondrogenically by growth and differentiation factor-5 （GDF-5） in vitro,then self-assembled to form three-dimensional structures. After 4 weeks of self-assembly, the tissue-engineered constructs were observed and detected morphologically, histologically and histochemically. Results The tissue-engineered constructs were hyaline-like in appearance, and had a conspicuous expression of collagen Ⅱ , aggrecan, GAG and proteoglycan. The mean absorbance value of collagen Ⅱ expression in GDF-5 group was （0. 1678 ± 0. 0222）, which was higher than that in control group （0.0908 ±0. 0145 ）（P 〈0. 05）. Conclusion The constructs had the histological, biochemical, and biomechanical properties approaching those of native articular cartilage. Self-assembled cultures appear promising for tissue engineering of cartilage.